[6]‐Gingerol Ameliorates ISO‐Induced Myocardial Fibrosis by Reducing Oxidative Stress, Inflammation, and Apoptosis through Inhibition of TLR4/MAPKs/NF‐κB Pathway
- 21 May 2020
- journal article
- research article
- Published by Wiley in Molecular Nutrition & Food Research
- Vol. 64 (13), e2000003
- https://doi.org/10.1002/mnfr.202000003
Abstract
Scope [6]‐gingerol is one of the primary pungent constituents of ginger. While [6]‐gingerol has many pharmacological effects, its benefits for myocardial fibrosis, including its exact role and underlying mechanisms, remain largely unexplored. The present study was designed to characterize the cardio‐protective effects of [6]‐gingerol in myocardial fibrosis mice and possible underlying mechanisms. Scope [6]‐gingerol is one of the primary pungent constituents of ginger. While [6]‐gingerol has many pharmacological effects, its benefits for myocardial fibrosis, including its exact role and underlying mechanisms, remain largely unexplored. The present study was designed to characterize the cardio‐protective effects of [6]‐gingerol in myocardial fibrosis mice and possible underlying mechanisms Methods and Results Mice were subcutaneously injected with isoproterenol (ISO, 10mg/kg) and gavaged with [6]‐gingerol (10, 20 mg/kg/day) for 14 days. Pathological alterations, fibrosis, oxidative stress, inflammation response, and apoptosis were examined. In ISO‐induced myocardial fibrosis, [6]‐gingerol treatment decreased the J‐point, heart rate, cardiac weight index (CWI), left ventricle weight index (LVWI), creatine kinase (CK) and lactate dehydrogenase (LDH) serum levels, calcium concentration, reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione disulfide (GSSG), increased levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and GSH/GSSG. Further, [6]‐gingerol improved ISO‐induced morphological pathologies, reduced the expression of TNF‐α, IL‐6, c‐fos, c‐jun, Bax, Caspase‐3, TLR4, NF‐κB, p38, p‐p38, ERK1/2, p‐ERK1/2, JNK, and p‐JNK, and increased Bcl‐2 protein expression. Conclusion The protective effect of [6]‐gingerol in mice with ISO‐induced myocardial fibrosis may be related to the inhibition of oxidative stress, inflammation, and apoptosis, potentially through the TLR4/MAPKs/NF‐κB signaling pathway. This article is protected by copyright. All rights reservedKeywords
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