Novel voltammetric tumor necrosis factor-alpha (TNF-α) immunosensor based on gold nanoparticles involved in thiol-functionalized multi-walled carbon nanotubes and bimetallic Ni/Cu-MOFs
- 5 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 413 (9), 2481-2492
- https://doi.org/10.1007/s00216-021-03203-z
Abstract
TNF-α, as a pro-inflammatory cytokine, regulates some physiological and pathological courses. TNF-α level increases in some important diseases such as cancer, arthritis, and diabetes. In addition, it displays an important function in Alzheimer’s and cardiovascular diseases. Herein, a novel, sensitive, and selective voltammetric TNF-α immunosensor was prepared by using gold nanoparticles involved in thiol-functionalized multi-walled carbon nanotubes (AuNPs/S-MWCNTs) as sensor platform and bimetallic Ni/Cu-MOFs as sensor amplification. Firstly, the sensor platform was developed on glassy carbon electrode (GCE) surface by using mixture of thiol-functionalized MWCNTs (S-MWCNTs) and AuNPs. Then, capture TNF-α antibodies were conjugated to sensor platform by amino-gold affinity. After capture TNF-α antibodies’ immobilization, a new-type voltammetric TNF-α immunosensor was developed by immune reaction between AuNPs/S-MWCNTs immobilized with primer TNF-α antibodies and bimetallic Ni/Cu-MOFs conjugated with seconder TNF-α antibodies. The prepared TNF-α immunosensor was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), thermogravimetric analysis, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). A linearity range of 0.01–1.0 pg mL−1 and a low detection limit of 2.00 fg mL−1 were also obtained for analytical applications.Keywords
This publication has 66 references indexed in Scilit:
- A novel voltammetric sensor based on p-aminothiophenol functionalized graphene oxide/gold nanoparticles for determining quercetin in the presence of ascorbic acidJournal of Electroanalytical Chemistry, 2013
- Sensitive immunosensor for tumor necrosis factor α based on dual signal amplification of ferrocene modified self-assembled peptide nanowire and glucose oxidase functionalized gold nanorodBiosensors and Bioelectronics, 2013
- Real-time label-free immunoassay of interferon-gamma and prostate-specific antigen using a Fiber-Optic Localized Surface Plasmon Resonance sensorBiosensors and Bioelectronics, 2013
- Direct electron transfer of glucose oxidase at glassy carbon electrode modified with functionalized carbon nanotubes within a dihexadecylphosphate filmSensors and Actuators B: Chemical, 2011
- TGFβ/TNFα-Mediated Epithelial–Mesenchymal Transition Generates Breast Cancer Stem Cells with a Claudin-Low PhenotypeCancer Research, 2011
- Localized Surface Plasmon Resonance SensorsChemical Reviews, 2011
- Development of a novel single sensor multiplexed marker assayThe Analyst, 2011
- Dual-color Proteomic Profiling of Complex Samples with a Microarray of 810 Cancer-related AntibodiesMolecular & Cellular Proteomics, 2010
- Cytokines, Inflammation, and PainInternational Anesthesiology Clinics, 2007
- Measurement of cytokine release at the single cell level using the ELISPOT assayMethods, 2006