Diversity of Ectomycorrhizal Fungal Communities in Four Types of Stands in Pinus massoniana Plantation in the West of China
Open Access
- 1 June 2021
- Vol. 12 (6), 719
- https://doi.org/10.3390/f12060719
Abstract
Ectomycorrhizal (ECM) fungi can form symbioses with plant roots, which play an important role in regulating the rhizosphere microenvironment. As a broad-spectrum ECM tree species, Pinus massoniana forms symbiotic relationship called mycorrhiza with various ECM fungal species. In this study, four types of forests were selected from a 38-year-old Pinus plantation in eastern Sichuan, namely, pure P. massoniana forest (MC), P. massoniana mixed with Cunninghamia lanceolata forest (MS), P. massoniana–Cryptomeria fortunei forest (ML), and P. massoniana–broadleaved forest (MK), the species mixture ratio of all forests was 1:1. The ITS2 segment of ECM root tip sequenced by high-throughput sequencing using the Illumina MiSeq sequencing platform. (1) The ECM fungi of these four P. massoniana forests showed similar dominant genera but different relative abundances in community structure during the three seasons. (2) The alpha diversity index of ECM fungi was significantly influenced by season and forest type. (3) Soil pH, soil organic matter (SOM), total nitrogen (TN), C/N ratio, and total phosphorus (TP) influenced the ECM fungal community structure in different seasons. In summary, there were significant differences in ECM fungal communities among different forest types and different seasons; the colonization rate of ECM fungal in P. massoniana–Cunninghamia lanceolata was the highest, so we infer that Cunninghamia lanceolata is the most suitable tree species for mixed with P. massoniana in three mixture forests.Keywords
Funding Information
- National Key Research and Development Program of China (2017YFD060030205)
- German Government loans for Sichuan. Forestry Sustainable Management (G1403083, 1922999002)
This publication has 44 references indexed in Scilit:
- Ectomycorrhizal‐fungal colonization induces physio‐morphological changes in Quercus serrata leaves and rootsJournal of Plant Nutrition and Soil Science, 2012
- Interactive metagenomic visualization in a Web browserBMC Bioinformatics, 2011
- Ectomycorrhizal fungal diversity in orchards of cultivated pecan (Carya illinoinensis; Juglandaceae)Mycorrhiza, 2011
- Distinct seasonal assemblages of arbuscular mycorrhizal fungi revealed by massively parallel pyrosequencingNew Phytologist, 2011
- Metagenomic biomarker discovery and explanationGenome Biology, 2011
- Potential link between plant and fungal distributions in a dipterocarp rainforest: community and phylogenetic structure of tropical ectomycorrhizal fungi across a plant and soil ecotoneNew Phytologist, 2009
- Dictionary of the FungiMycological Research, 2009
- Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial TaxonomyApplied and Environmental Microbiology, 2007
- Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARBApplied and Environmental Microbiology, 2006
- The magnitude of fungal diversity: the 1.5 million species estimate revisitedMycological Research, 2001