Advances in Exosome Analysis Methods with an Emphasis on Electrochemistry
- 9 September 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 92 (19), 12733-12740
- https://doi.org/10.1021/acs.analchem.0c02745
Abstract
Exosomes, small extracellular vesicles, are released by various cell types. They are found in bodily fluids, including blood, urine, serum, and saliva, and play essential roles in intercellular communication. Exosomes contain various biomarkers, such as nucleic acids and proteins, that reflect the status of their parent cells. Since they influence tumorigenesis and metastasis in cancer patients, exosomes are excellent non-invasive potential indicators for early cancer detection. Aptamers with specific binding properties have distinct advantages over antibodies, making them effective versatile bioreceptors for the detection of exosome biomarkers. Here, we review various aptamer-based exosome detection approaches based on signaling methods, such as fluorescence, colorimetry, and chemiluminescence, focusing on electrochemical strategies that are easier, cost effective, and more sensitive than others. Further, we discuss the clinical applications of electrochemical exosome analysis strategies as well as future research directions in this field.Keywords
Funding Information
- National Research Foundation of Korea (NRF-2020R1C1C1012275)
This publication has 105 references indexed in Scilit:
- A comprehensive overview of exosomes as drug delivery vehicles — Endogenous nanocarriers for targeted cancer therapyBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 2014
- Double-stranded DNA in exosomes: a novel biomarker in cancer detectionCell Research, 2014
- In-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteomeBiochemical and Biophysical Research Communications, 2014
- Exosomes: the future of biomarkers in medicineBiomarkers in Medicine, 2013
- Electrochemical impedance spectroscopy: an overview of bioanalytical applicationsAnalytical Methods, 2013
- Trash or Treasure: extracellular microRNAs and cell-to-cell communicationFrontiers in Genetics, 2013
- Study of Electrode Reactions and Interfacial PropertiesPublished by Wiley ,2006
- Electrochemical SensorsPublished by Wiley ,2006
- Controlled‐Potential TechniquesPublished by Wiley ,2006
- Fundamental ConceptsPublished by Wiley ,2006