3D‐bulk to nanoforms of modified hydroxyapatite: Characterization and osteogenic potency in an in vitro 3D bone model system
- 17 December 2021
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part B: Applied Biomaterials
- Vol. 110 (5), 1151-1164
- https://doi.org/10.1002/jbm.b.34989
Abstract
Synthetic bioceramics are replacing conventional methods of treating bone defects with autografts owing to the high demand of bone substitutes, with their Surface topography and size contributing to favor cytocompatibility in tissue regeneration. This experimental study deals with the comparative evaluation of the physical characterizations of four different in-house synthesized bioceramics from 3D-bulk to nanoforms of hydroxyapatite (HA), Biphasic calcium phosphate (BCP), Strontium doped hydroxyapatite (SrHA) and Silica coated hydroxyapatite (HASi) and also simultaneously evaluates adhesion, proliferation and osteogenic differentiation of rabbit adipose derived mesenchymal stem cells (RADMSCs) on these biomimetic ceramic niches. The osteogenic induced cells grown on 3D scaffolds for a period of 7, 14, 21, and 28 days were analyzed for their viability (MTT, LDH, live-dead assays), morphology (SEM), proliferation (Cytox-Red) and osteogenic differentiation (ALP, osteocalcin expression). Cellular activities and differentiation of RADMSCs were significantly higher on SrHA indicating the role of strontium in the differentiation of mesenchymal stem cells on this ceramic platform to the bone lineage. In order to reinforce the materials for hard tissue implantation and drug delivery, nano-SrHA (nSrHA) became the nanoparticle of choice based on its non-toxicity, cytocompatibility and osteogenic properties (nSrHA > nHASi > nBCP > nHA).Keywords
This publication has 41 references indexed in Scilit:
- Tailoring the Nanostructured Surfaces of Hydroxyapatite Bioceramics to Promote Protein Adsorption, Osteoblast Growth, and Osteogenic DifferentiationACS Applied Materials & Interfaces, 2013
- Current Application of β‐tricalcium Phosphate Composites in OrthopaedicsOrthopaedic Surgery, 2012
- Strontium Enhances Osteogenic Differentiation of Mesenchymal Stem Cells and In Vivo Bone Formation by Activating Wnt/Catenin SignalingThe International Journal of Cell Cloning, 2011
- Highly Extensible, Tough, and Elastomeric Nanocomposite Hydrogels from Poly(ethylene glycol) and Hydroxyapatite NanoparticlesBiomacromolecules, 2011
- Evolution of bioceramics within the field of biomaterialsComptes Rendus. Chimie, 2010
- Strontium Promotes Osteogenic Differentiation of Mesenchymal Stem Cells Through the Ras/MAPK Signaling PathwayCellular Physiology and Biochemistry, 2009
- Biodegradation and Cytocompatibility Studies of a Triphasic Ceramic‐Coated Porous Hydroxyapatite for Bone Substitute ApplicationsInternational Journal of Applied Ceramic Technology, 2008
- Comparison of osteoblast‐like cell responses to calcium silicate and tricalcium phosphate ceramics in vitroJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2006
- Bone Tissue Engineering: State of the Art and Future TrendsMacromolecular Bioscience, 2004
- In vivo cancellous bone remodeling on a strontium‐containing hydroxyapatite (sr‐HA) bioactive cementJournal of Biomedical Materials Research Part A, 2003