Programming DNA‐Based Systems through Effective Molarity Enforced by Biomolecular Confinement
- 19 May 2020
- journal article
- review article
- Published by Wiley in Chemistry – A European Journal
- Vol. 26 (44), 9826-9834
- https://doi.org/10.1002/chem.202001660
Abstract
The fundamental concept of effective molarity is observed in a variety of biological processes such as protein compartmentalization within organelles, membrane localization and signaling paths. To control molecular encountering and promote effective interactions, Nature places biomolecules in specific sites inside the cell in order to generate a high, localized concentration different from the bulk concentration. Inspired by this mechanism, scientists have artificially recreated in the lab the same strategy to actuate and control artificial DNA‐based functional systems. Here we discuss how harnessing effective molarity has led to the development of a number of proximity‐induced strategies with applications ranging from DNA‐templated organic chemistry and catalysis, to biosensing and protein‐supported DNA assembly.Funding Information
- H2020 Marie Skłodowska-Curie Actions (778133, 843998, 765703)
- Fondazione Umberto Veronesi
This publication has 125 references indexed in Scilit:
- Reversible blocking of antibodies using bivalent peptide–DNA conjugates allows protease-activatable targetingChemical Science, 2013
- Dynamic DNA Assemblies Mediated by Binding-Induced DNA Strand DisplacementJournal of the American Chemical Society, 2013
- Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA NanostructuresJournal of the American Chemical Society, 2012
- Oligonucleotide-Templated Reactions for Sensing Nucleic AcidsMolecules, 2012
- A DNA-based molecular motor that can navigate a network of tracksNature Nanotechnology, 2012
- Autonomous multistep organic synthesis in a single isothermal solution mediated by a DNA walkerNature Nanotechnology, 2010
- DNA-Templated Synthesis of Trimethine Cyanine Dyes: A Versatile Fluorogenic Reaction for Sensing G-Quadruplex FormationAngewandte Chemie, 2010
- Antigen Peptide-Based Immunosensors for Rapid Detection of Antibodies and AntigensAnalytical Chemistry, 2009
- Direct observation of individual endogenous protein complexes in situ by proximity ligationNature Methods, 2006
- Protein detection using proximity-dependent DNA ligation assaysNature Biotechnology, 2002