Impact of taurine on red blood cell metabolism and implications for blood storage
- 27 April 2020
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 60 (6), 1212-1226
- https://doi.org/10.1111/trf.15810
Abstract
BACKGROUND Taurine is an antioxidant that is abundant in some common energy drinks. Here we hypothesized that the antioxidant activity of taurine in red blood cells (RBCs) could be leveraged to counteract storage‐induced oxidant stress. STUDY DESIGN AND METHODS Metabolomics analyses were performed on plasma and RBCs from healthy volunteers (n = 4) at baseline and after consumption of a whole can of a common, taurine‐rich (1000 mg/serving) energy drink. Reductionistic studies were also performed by incubating human RBCs with taurine ex vivo (unlabeled or 13C15N‐labeled) at increasing doses (0, 100, 500, and 1000 μmol/L) at 37°C for up to 16 hours, with and without oxidant stress challenge with hydrogen peroxide (0.1% or 0.5%). Finally, we stored human and murine RBCs under blood bank conditions in additives supplemented with 500 μmol/L taurine, before metabolomics and posttransfusion recovery studies. RESULTS Consumption of energy drinks increased plasma and RBC levels of taurine, which was paralleled by increases in glycolysis and glutathione (GSH) metabolism in the RBC. These observations were recapitulated ex vivo after incubation with taurine and hydrogen peroxide. Taurine levels in the RBCs from the REDS‐III RBC‐Omics donor biobank were directly proportional to the total levels of GSH and glutathionylated metabolites and inversely correlated to oxidative hemolysis measurements. Storage of human RBCs in the presence of taurine improved energy and redox markers of storage quality and increased posttransfusion recoveries in FVB mice. CONCLUSION Taurine modulates RBC antioxidant metabolism in vivo and ex vivo, an observation of potential relevance to transfusion medicine.Keywords
Funding Information
- National Heart, Lung, and Blood Institute (HHSN2682011, R01HL146442, R01HL148151)
- National Institute of General Medical Sciences (RM1GM131968)
This publication has 63 references indexed in Scilit:
- Time-course investigation of SAGM-stored leukocyte-filtered red bood cell concentrates: from metabolism to proteomicsHaematologica, 2011
- Mechanism underlying the antioxidant activity of taurine: prevention of mitochondrial oxidant productionAmino Acids, 2011
- Peroxiredoxin‐2 as a candidate biomarker to test oxidative stress levels of stored red blood cells under blood bank conditionsTransfusion, 2011
- Glucose-6-phosphate Dehydrogenase Deficiency and Malaria: Cytochemical Detection of Heterozygous G6PD Deficiency in WomenJournal of Histochemistry & Cytochemistry, 2009
- Role of antioxidant activity of taurine in diabetesThis article is one of a selection of papers from the NATO Advanced Research Workshop on Translational Knowledge for Heart Health (published in part 1 of a 2-part Special Issue).Canadian Journal of Physiology and Pharmacology, 2009
- Progressive oxidation of cytoskeletal proteins and accumulation of denatured hemoglobin in stored red cellsJournal of Cellular and Molecular Medicine, 2007
- Taurine increases mitochondrial buffering of calcium: role in neuroprotectionAmino Acids, 2006
- Effect of lipid peroxides and antioxidants on glycation of hemoglobin: An in vitro study on human erythrocytesClinica Chimica Acta; International Journal of Clinical Chemistry, 2006
- The Protective Effect of Taurine on the Biomembrane against Damage Produced by Oxygen Radicals.Biological & Pharmaceutical Bulletin, 1993
- Einige neue Bestandtheile der Galle des OchsenAnnalen der Physik, 1827