Collection and storage of DNA-containing biomaterial and isolated DNA
Open Access
- 31 December 2020
- journal article
- Published by Silicea - Poligraf, LLC in Cardiovascular Therapy and Prevention
- Vol. 19 (6), 2730
- https://doi.org/10.15829/1728-8800-2020-2730
Abstract
The advances of biomedicine include the new technologies, diagnosis and treatment techniques, as well as the practical use of new types of biological targets, in particular, nucleic acids. Genomic deoxyribonucleic acid (DNA), extracellular DNA (exDNA) and microbiome DNA obtained from different types of samples (tissues, blood and its derivatives, feces, etc.) are used as objects of genetic research. The use of new technologies for DNA analysis required the development of standardized methods for processing biological samples in order to obtain high-quality DNA samples. The research uses various methods for collecting, preparing samples and storing various DNA-containing biomaterials and isolated DNA, as well as methods for assessing the quality of samples and biobank standards. It is obvious that the use of uniform standards will allow large-scale genetic research on the basis of biobanks and research laboratories. Specialists from professional organizations such as International Society for Biological and Environmental Repositories (ISBER), Biobanking and BioMolecular Resources Research Infrastructure-European Research Infrastructure Consortium (BBMRI-ERIC), European, Middle Eastern & African Society for Biopreservationa and Biobanking (ESBB) and the Russian National Association of Biobanks and Biobanking Professionals.Keywords
This publication has 46 references indexed in Scilit:
- Cell-free DNA: Preanalytical variablesClinica Chimica Acta; International Journal of Clinical Chemistry, 2015
- Gut Microbiota Dysbiosis in Obesity-Linked Metabolic Diseases and Prebiotic Potential of Polyphenol-Rich ExtractsCurrent Obesity Reports, 2015
- The MicrobiomeVeterinary Pathology, 2015
- Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolationBMC Genomics, 2015
- Effect of preservation method on spider monkey (Ateles geoffroyi) fecal microbiota over 8weeksJournal of Microbiological Methods, 2015
- The Effect of Sampling and Storage on the Fecal Microbiota Composition in Healthy and Diseased SubjectsPLOS ONE, 2015
- The Effects of Freezing on Faecal Microbiota as Determined Using MiSeq Sequencing and Culture-Based InvestigationsPLOS ONE, 2015
- Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differencesFrontiers in Microbiology, 2015
- Dysbiosis of the gut microbiota in diseaseMicrobial Ecology in Health & Disease, 2015
- Function and information content of DNA methylationNature, 2015