Effects of functional polysaccharide from silkworm as an immunostimulant on transcriptional profiling and disease resistance in fish
Open Access
- 28 October 2022
- journal article
- research article
- Published by Brill in Journal of Insects as Food and Feed
- Vol. 8 (11), 1221-1233
- https://doi.org/10.3920/jiff2021.0108
Abstract
Dietary manipulation to maintain fish health and reduce bacterial infection through the use of immunostimulants has been widely used worldwide. A broad range of bioactive substances capable of optimising animal health has been found in several insect species, including antimicrobial/antiviral peptides, polysaccharides such as chitin, lauric acid, and insect products such as honey. Recently, we identified a novel bioactive polysaccharide from Bombyx mori, termed silkrose-BM, that can activate innate immunity in mammalian RAW264.7 macrophages and provide effective protection against vibriosis in penaeid prawns. However, the efficacy of dietary silkrose-BM in teleosts remains unclear. Here, we investigated the effects of dietary inclusion of silkrose-BM in Japanese medaka (Oryzias latipes) after they were artificially challenged with Edwardsiella tarda. The survival of medaka after infection with E. tarda was significantly improved by dietary silkrose-BM at a concentration of 10, 100, and 1000 ng/g. RNA-seq analysis was performed in the intestine and liver of the medaka to identify changes in the transcriptional profiling evoked by silkrose-BM. The dietary silkrose-BM group showed 1,194 and 2,259 differentially expressed genes (DEGs) in the intestine and liver, respectively, when compared with the control group prior to E. tarda infection. Functional enrichment analysis of DEGs showed several putative genes involved in the Toll-like receptor/nuclear factor κB pathway, cytokine-cytokine receptor interactions, complement cascade, antimicrobial peptides, and junctional modification. Taken together, these results suggest that silkrose-BM used as an immunostimulant can improve the immune system and resistance to edwardsiellosis in teleosts.Keywords
This publication has 69 references indexed in Scilit:
- Inflammatory responses in primary muscle cell cultures in Atlantic salmon (Salmo salar)BMC Genomics, 2013
- TCC: an R package for comparing tag count data with robust normalization strategiesBMC Bioinformatics, 2013
- Immune-responsive gene 1 protein links metabolism to immunity by catalyzing itaconic acid productionProceedings of the National Academy of Sciences of the United States of America, 2013
- Pathogenesis of and strategies for preventing Edwardsiella tarda infection in fishVeterinary Research, 2012
- clusterProfiler: an R Package for Comparing Biological Themes Among Gene ClustersOMICS: A Journal of Integrative Biology, 2012
- The role of pattern-recognition receptors in innate immunity: update on Toll-like receptorsNature Immunology, 2010
- The Sequence Alignment/Map format and SAMtoolsBioinformatics, 2009
- Chitin regulation of immune responses: an old molecule with new rolesCurrent Opinion in Immunology, 2008
- Pathogen Recognition and Innate ImmunityCell, 2006
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001