Influenza A variants with reduced susceptibility to baloxavir isolated from Japanese patients are fit and transmit through respiratory droplets
Top Cited Papers
- 25 November 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Microbiology
- Vol. 5 (1), 27-33
- https://doi.org/10.1038/s41564-019-0609-0
Abstract
Here we report the isolation of the influenza A/H1N1 2009 pandemic (A/H1N1pdm) and A/H3N2 viruses carrying an I38T mutation in the polymerase acidic protein—a mutation that confers reduced susceptibility to baloxavir marboxil—from patients before and after treatment with baloxavir marboxil in Japan. These variants showed replicative abilities and pathogenicity that is similar to those of wild-type isolates in hamsters; they also transmitted efficiently between ferrets by respiratory droplets.Keywords
Funding Information
- Japan Agency for Medical Research and Development (JP18am001007, JP19fk0108056, JP18am001007, JP19fk0108066, JP19fk0108058, JP18am001007, JP19fk0108031, JP19fk0108056, JP19fm0108006)
- Ministry of Education, Culture, Sports, Science and Technology (16H06429, 16K21723, 16H06434)
- U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (CRIP, HHSN272201400008C)
This publication has 21 references indexed in Scilit:
- Least-Squares Means: TheRPackagelsmeansJournal of Statistical Software, 2016
- Fitting Linear Mixed-Effects Models Usinglme4Journal of Statistical Software, 2015
- H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory DropletScience, 2013
- Pathogenesis and transmission of avian influenza A (H7N9) virus in ferrets and miceNature, 2013
- Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferretsNature, 2012
- Cutadapt removes adapter sequences from high-throughput sequencing readsEMBnet.Journal, 2011
- Fast and accurate long-read alignment with Burrows–Wheeler transformBioinformatics, 2010
- Transmission and Pathogenesis of Swine-Origin 2009 A(H1N1) Influenza Viruses in Ferrets and MiceScience, 2009
- Fast and accurate short read alignment with Burrows–Wheeler transformBioinformatics, 2009
- Efficient selection for high-expression transfectants with a novel eukaryotic vectorGene, 1991