Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China
Open Access
- 8 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Parasites & Vectors
- Vol. 13 (1), 1-10
- https://doi.org/10.1186/s13071-020-04046-w
Abstract
BackgroundEnterocytozoon bieneusi is one of common intestinal pathogens in humans and animals including non-human primates (NHPs). Many zoonotic pathogens including E. bieneusi have been found in these animals. However, there are few studies on the population structure of E. bieneusi in NHPs. To infer the gene diversity and population genetics of E. bieneusi, we selected 88 E. bieneusi-positive samples from crab-eating macaques for multilocus characterizations in this study.MethodsThe E. bieneusi isolates examined belonged to three common genotypes with different host ranges by sequence analysis of the ribosomal internal transcribed spacer (ITS): Type IV (n=44), Macaque3 (n=24) and Peru8 (n=20). They were further characterized by sequence analysis at four microsatellite and minisatellite loci (MS1, MS3, MS4 and MS7). DnaSP, Arlequin and LIAN were used to analyze the sequence data together with those from the ITS locus to infer the population genetics. Subpopulation structure was inferred using phylogenetic and STRUCTURE analyses.ResultsSeventy-two (81.8%), 71 (80.7%), 76 (86.4%) and 79 (89.8%) samples were amplified and sequenced successfully at the MS1, MS3, MS4 and MS7 loci, respectively, with 53 having sequence data at all five MLST loci including ITS. Altogether, 33 multilocus genotypes (MLGs) were produced based on concatenated sequences from the 53 samples. In phylogenetic analyses of sequences and allelic data, four major subpopulations (SPs) were observed with different ITS genotypes in each of them: Type IV and Peru8 in SP1 and SP2; Type IV, Macaque3 and Peru8 in SP3; and Type IV and Macaque3 in SP4. SP3 and SP4 were phylogenetically related and might be NHP-specific based on the fact that Macaque3 is mostly found in NHPs. A strong linkage disequilibrium (LD) was observed among the multilocus sequences and allelic data.ConclusionsThe significant LD in the multilocus sequence analysis indicated the presence of an overall clonal population structure of E. bieneusi in crab-eating macaques. The inconsistent segregation of MLGs among ITS genotypes suggested some occurrence of genetic recombination. These observations should improve our understanding of the population genetics of E. bieneusi in NHPs.Keywords
Funding Information
- National Key R&D Program of China (2017YFC1601206)
- National Natural Science Foundation of China (31820103014)
This publication has 54 references indexed in Scilit:
- Long-Term Monitoring of Microsporidia, Cryptosporidium and Giardia Infections in Western Lowland Gorillas (Gorilla gorilla gorilla) at Different Stages of Habituation in Dzanga Sangha Protected Areas, Central African RepublicPLOS ONE, 2013
- Epidemiology ofEnterocytozoon bieneusiInfection in HumansJournal of Parasitology Research, 2012
- Cyclospora papionis, Cryptosporidium hominis, and Human-Pathogenic Enterocytozoon bieneusi in Captive Baboons in KenyaJournal of Clinical Microbiology, 2011
- MicrosporidiosisCurrent Opinion in Infectious Diseases, 2011
- Development of a Multilocus Sequence Typing Tool for High-Resolution Genotyping of Enterocytozoon bieneusiApplied and Environmental Microbiology, 2011
- Microsporidiosis: Enterocytozoon bieneusi in domesticated and wild animalsResearch in Veterinary Science, 2011
- Identification and Genotyping of Enterocytozoon bieneusi in ChinaJournal of Clinical Microbiology, 2011
- Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and WindowsMolecular Ecology Resources, 2010
- Enterocytozoon bieneusi Genotype Nomenclature Based on the Internal Transcribed Spacer Sequence: A ConsensusThe Journal of Eukaryotic Microbiology, 2009
- Occurrence of a New Microsporidan: Enterocytozoon bieneusi n. g., n. sp., in the Enterocytes of a Human Patient with AIDS1The Journal of Protozoology, 1985