Resistance training increases muscle NAD+ and NADH concentrations as well as NAMPT protein levels and global sirtuin activity in middle-aged, overweight, untrained individuals
Open Access
- 31 May 2020
- journal article
- research article
- Published by Impact Journals, LLC in Aging
- Vol. 12 (10), 9447-9460
- https://doi.org/10.18632/aging.103218
Abstract
We examined if resistance training affected muscle NAD+ and NADH concentrations as well as nicotinamide phosphoribosyltransferase (NAMPT) protein levels and sirtuin (SIRT) activity markers in middle-aged, untrained (MA) individuals. MA participants (59 +/- 4 years old; n=16) completed 10 weeks of full-body resistance training ( 2 d/wk). Body composition, knee extensor strength, and vastus lateralis muscle biopsies were obtained prior to training (Pre) and 72 hours following the last training bout (Post). Data from trained college-aged men (22 +/- 3 years old, training age: 6 +/- 2 years old; n=15) were also obtained for comparative purposes. Muscle NAD+ (+127%, p<0.001), NADH (+99%, p=0.002), global SIRT activity (+13%, p=0.036), and NAMPT protein (+15%, p=0.014) increased from Pre to Post in MA participants. Additionally, Pre muscle NAD(+) and NADH in MA participants were lower than college-aged participants (p0.10). Interestingly, muscle citrate synthase activity levels (i.e., mitochondrial density) increased in MA participants from Pre to Post (+183%, p<0.001), and this increase was significantly associated with increases in muscle NAD(+) (r(2)=0.592, p=0.001). In summary, muscle NAD+, NADH, and global SIRT activity are positively affected by resistance training in middle-aged, untrained individuals. Whether these adaptations facilitated mitochondrial biogenesis remains to be determined.Keywords
This publication has 63 references indexed in Scilit:
- Age Related Changes in NAD+ Metabolism Oxidative Stress and Sirt1 Activity in Wistar RatsPLOS ONE, 2011
- Structural basis for sirtuin function: What we know and what we don'tBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2010
- SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylationNature, 2010
- Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged ratsMechanisms of Ageing and Development, 2010
- Skeletal muscle NAMPT is induced by exercise in humansAmerican Journal of Physiology-Endocrinology and Metabolism, 2010
- SIRT1 Controls the Transcription of the Peroxisome Proliferator-activated Receptor-γ Co-activator-1α (PGC-1α) Gene in Skeletal Muscle through the PGC-1α Autoregulatory Loop and Interaction with MyoDJournal of Biological Chemistry, 2009
- Circadian Clock Feedback Cycle Through NAMPT-Mediated NAD + BiosynthesisScience, 2009
- Glucose Restriction Inhibits Skeletal Myoblast Differentiation by Activating SIRT1 through AMPK-Mediated Regulation of NamptDevelopmental Cell, 2008
- Mammalian Sir2 Homolog SIRT3 Regulates Global Mitochondrial Lysine AcetylationMolecular and Cellular Biology, 2007
- Metabolic control of muscle mitochondrial function and fatty acid oxidation through SIRT1/PGC-1αThe EMBO Journal, 2007