Heat stress interferes with chromosome segregation and cytokinesis during male meiosis in Arabidopsis thaliana
- 3 May 2020
- journal article
- research article
- Published by Taylor & Francis Ltd in Plant Signaling & Behavior
- Vol. 15 (5), 1746985
- https://doi.org/10.1080/15592324.2020.1746985
Abstract
In higher plants, male meiosis is a key process of microsporogenesis and is crucial for plant fertility. Male meiosis programs are prone to be influenced by altered temperature conditions. Studies have reported that an increased temperature (28 degrees C) within a fertile threshold can affect the frequency of meiotic recombination in Arabidopsis. However, not much has been known how male meiosis responses to an extremely high temperature beyond the fertile threshold. To understand the impact of extremely high temperature on male meiosis in Arabidopsis, we treated flowering Arabidopsis plants with 36-38 degrees C and found that the high-temperature condition significantly reduced pollen shed and plant fertility, and led to formation of pollen grains with varied sizes. The heat stress-induced unbalanced tetrads, polyad and meiotic restitution, suggesting that male meiosis was interfered. Fluorescence in situ hybridization (FISH) assay confirmed that both homologous chromosome separation and sister chromatids cohesion were influenced. Aniline blue staining of tetrad-stage pollen mother cells (PMCs) revealed that meiotic cytokinesis was severely disrupted by the heat stress. Supportively, immunolocalization of -tubulin showed that the construction of spindle and phragmoplast at both meiosis I and II were interfered. Overall, our findings demonstrate that an extremely high-temperature stress over the fertile threshold affects both chromosome segregation and cytokinesis during male meiosis by disturbing microtubular cytoskeleton in Arabidopsis.This publication has 63 references indexed in Scilit:
- Production of Diploid Male Gametes in Arabidopsis by Cold-Induced Destabilization of Postmeiotic Radial Microtubule ArraysPlant Physiology, 2012
- The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3PLoS Genetics, 2012
- The Recombinases DMC1 and RAD51 Are Functionally and Spatially Separated during Meiosis in ArabidopsisTHE PLANT CELL ONLINE, 2012
- HINKEL kinesin, ANP MAPKKKs and MKK6/ANQ MAPKK, which phosphorylates and activates MPK4 MAPK, constitute a pathway that is required for cytokinesis in Arabidopsis thalianaPlant and Cell Physiology, 2010
- Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen GrainsPLoS Genetics, 2008
- The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in ArabidopsisTHE PLANT CELL ONLINE, 2007
- ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in ArabidopsisGenes & Development, 2007
- Pollen tetrad-based visual assay for meiotic recombination in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2007
- Visualization of microtubules in living cells of transgenicArabidopsis thalianaProtoplasma, 1999
- Isolation and Characterization of SYN1, a RAD21-like Gene Essential for Meiosis in ArabidopsisTHE PLANT CELL ONLINE, 1999