Identification of Functional Single Nucleotide Polymorphisms in Porcine HSD17B14 Gene Associated with Estrus Behavior Difference between Large White and Mi Gilts
Open Access
- 11 November 2020
- journal article
- research article
- Published by MDPI AG in Biomolecules
- Vol. 10 (11), 1545
- https://doi.org/10.3390/biom10111545
Abstract
Steroid hormone levels are associated with estrous behavior, which affects timely mating and reproductive efficiency in pigs. 17β-hydroxysteroid dehydrogenase type 14 (HSD17B14) modulates steroid synthesis and metabolism. To identify the functional single nucleotide polymorphisms (SNPs) in the porcine HSD17B14 gene, ear tissues from Large White and Mi gilts were collected to extract genomic DNA. Variable lengths of truncated promoter of HSD17B14 gene were used to determine the promoter activity by a dual luciferase reporter system. The vector HSD17B14Phe or HSD17B14Val was transfected into porcine granulosa cells (GCs). The core promoter region was identified between −72 bp and −218 bp. Six of seven SNPs had significant differences of allele frequency between Large White and Mi gilts. The plasmids with the wild genotype AA of rs329427898 maintained a smaller fraction of promoter activity compared with the plasmids with the mutant genotype GG, while the plasmids with wild the genotype TT of rs319864566 had a greater promoter activity than the plasmids with the mutant genotype CC. A missense mutation (Phe73Val) caused changes in the structural dynamics and function of the HSD17B14 protein. The highly expressed HSD17B14Val degraded less estradiol into estrone, while the relatively lowly expressed HSD17B14Phe degraded more estradiol into estrone, suggesting the protein activity of HSD17B14Phe was greater than that of HSD17B14Val. Moreover, the HSD17B14Phe group has a greater apoptosis rate of porcine GCs. The HSD17B14 gene could been used as a candidate molecular marker for estrus behavior in pigs.This publication has 52 references indexed in Scilit:
- CpG islands and the regulation of transcriptionGenes & Development, 2011
- Phenotypic and genetic correlations between gilt estrus, puberty, growth, composition, and structural conformation traits with first-litter reproductive measuresJournal of Animal Science, 2011
- Comparison of sow farrowing characteristics between a Chinese breed and three French breedsLivestock Science, 2009
- Functional characterization of the human TPH2 5′ regulatory region: untranslated region and polymorphisms modulate gene expression in vitroHuman Genetics, 2007
- Structural and biochemical characterization of human orphan DHRS10 reveals a novel cytosolic enzyme with steroid dehydrogenase activityBiochemical Journal, 2007
- The General Transcription Machinery and General CofactorsCritical Reviews in Biochemistry and Molecular Biology, 2006
- Environment, lifestyle and infertility — an inter-generational issueNature, 2002
- Patterns of linkage disequilibrium in the human genomeNature Reviews Genetics, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Gonadotropin Secretion in Ovariectomized Chinese Meishan and Hybrid Large White Gilts; Responses to Challenges with Estradiol Benzoate, Gonadotropin-Releasing Hormone, or Porcine Follicular Fluid1Biology of Reproduction, 1994