A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
Open Access
- 6 December 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Data
- Vol. 6 (1), 1-12
- https://doi.org/10.1038/s41597-019-0320-2
Abstract
The reliable taxonomic identification of organisms through DNA sequence data requires a well parameterized library of curated reference sequences. However, it is estimated that just 15% of described animal species are represented in public sequence repositories. To begin to address this deficiency, we provide DNA barcodes for 1,500,003 animal specimens collected from 23 terrestrial and aquatic ecozones at sites across Canada, a nation that comprises 7% of the planet’s land surface. In total, 14 phyla, 43 classes, 163 orders, 1123 families, 6186 genera, and 64,264 Barcode Index Numbers (BINs; a proxy for species) are represented. Species-level taxonomy was available for 38% of the specimens, but higher proportions were assigned to a genus (69.5%) and a family (99.9%). Voucher specimens and DNA extracts are archived at the Centre for Biodiversity Genomics where they are available for further research. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, and the Global Genome Biodiversity Network Data Portal.Keywords
Funding Information
- Ontario Ministry of Research, Innovation and Science
- Global Genome Biodiversity Network - Global Genome Initiative
- Ontario Ministry of Research, Innovation and Science
- Canada First Research Excellence Fund
- Canada Foundation for Innovation
- Genome Canada
- Ontario Genomics
- Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada
- Gordon and Betty Moore Foundation
- Ontario Ministry of Research, Innovation and Science
- Ann McCain Evans and Chris Evans
This publication has 66 references indexed in Scilit:
- Spiders (Araneae) of Churchill, Manitoba: DNA barcodes and morphology reveal high species diversity and new Canadian recordsBMC Ecology, 2013
- The diversity and biogeography of the Coleoptera of Churchill: insights from DNA barcodingBMC Ecology, 2013
- The effect of sampling from subdivided populations on species identification with DNA barcodes using a Bayesian statistical approachMolecular Phylogenetics and Evolution, 2012
- Environmental DNAMolecular Ecology, 2012
- When species matches are unavailable are DNA barcodes correctly assigned to higher taxa? An assessment using sphingid mothsBMC Ecology, 2011
- Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, CanadaFrontiers in Zoology, 2009
- Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplifiedProceedings of the National Academy of Sciences of the United States of America, 2008
- GenBankNucleic Acids Research, 2007
- bold: The Barcode of Life Data System (http://www.barcodinglife.org)Molecular Ecology Notes, 2007
- DNA Barcoding: Error Rates Based on Comprehensive SamplingPLoS Biology, 2005