Conductive Composite Fiber with Optimized Alignment Guides Neural Regeneration under Electrical Stimulation
- 31 January 2021
- journal article
- research article
- Published by Wiley in Advanced Healthcare Materials
- Vol. 10 (3), e2000604
- https://doi.org/10.1002/adhm.202000604
Abstract
Conductivity and alignment of scaffolds are two primary factors influencing the efficacy of nerve repair. Herein, conductive composite fibers composed of poly(e-caprolactone) (PCL) and carbon nanotubes (CNTs) with different orientation degrees are prepared by electrospinning at various rotational speeds (0, 500, 1000, and 2000 rpm), and meanwhile the synergistic promotion mechanism of aligned topography and electrical stimulation on neural regeneration is fully demonstrated. Under an optimized rotational speed of 1000 rpm, the electrospun PCL fiber exhibits orientated structure at macroscopic (mean deviation angle = 2.78 degrees) or microscopic crystal scale (orientation degree = 0.73), decreased contact angle of 99.2 degrees +/- 4.9 degrees, and sufficient tensile strength in both perpendicular and parallel directions to fiber axis (1.13 +/- 0.15 and 5.06 +/- 0.98 MPa). CNTs are introduced into the aligned fiber for further improving conductivity (15.69-178.63 S m(-1)), which is beneficial to the oriented growth of neural cells in vitro as well as the regeneration of injured sciatic nerves in vivo. On the basis of robust cell induction behavior, optimum sciatic nerve function index, and enhanced remyelination/axonal regeneration, such conductive PCL/CNTs composite fiber with optimized fiber alignment may serve as instructive candidates for promoting the scaffold- and cell-based strategies for neural repair.Funding Information
- National Natural Science Foundation of China (52022095, 51903050, 51873207, 51673187)
- Natural Science Foundation of Fujian Province (2019J01258)
This publication has 42 references indexed in Scilit:
- Centrifugal Spinning: An Alternative Approach to Fabricate Nanofibers at High Speed and Low CostPolymer Reviews, 2014
- Molecular weight-modulated electrospun poly(ε-caprolactone) membranes for postoperative adhesion preventionRSC Advances, 2014
- Neural tissue engineering options for peripheral nerve regenerationBiomaterials, 2014
- Tough and flexible CNT–polymeric hybrid scaffolds for engineering cardiac constructsBiomaterials, 2014
- Aligned neurite outgrowth and directed cell migration in self-assembled monodomain gelsBiomaterials, 2014
- End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regenerationBiomaterials, 2012
- Effect of carbon nanotube coating of aligned nanofibrous polymer scaffolds on the neurite outgrowth of PC‐12 cellsCell Biology International, 2011
- Development of carbon nanofibers from aligned electrospun polyacrylonitrile nanofiber bundles and characterization of their microstructural, electrical, and mechanical propertiesPolymer, 2009
- Electrospun poly(ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineeringBiomaterials, 2008
- From the membrane to the nucleus and back again: bifunctional focal adhesion proteinsCurrent Opinion in Cell Biology, 2006