Objectives: The aim of present investigation is to evaluate secondary metabolites in leaves, roots, and stem of Cleome viscosa L. qualitatively and quantitatively. Materials and Methods: The leaf, root, and stem powder samples were subjected to methanolic, ethanolic, and aqueous extraction using Soxhlet apparatus. The extracts were evaluated for major phytochemicals qualitatively and quantitatively using standard methods. Results: The phytochemical screening revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, saponins, and tannins. Anthocyanides and terpenoids were not detected in any of the extracts. Quantitative evaluation of leaves, roots, and stem extracts showed the alkaloids, cardiac glycosides, flavonoids, phenols, and saponins in significant amounts. Conclusion: The extracts of leaves, roots, and stem showed the presence of important secondary metabolites which have tremendous medicinal values. Thus, the use of this plant in ayurvedic system of medicines can be correlated with the modern-day science which is based on phytoconstituents.