A Candidate Prognostic Biomarker Complement Factor I Promotes Malignant Progression in Glioma
Open Access
- 4 February 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
Objectives: Glioma is the most common and aggressive type of primary central nervous system (CNS) tumor in adults and is associated with substantial mortality rates. The aim of our study was to evaluate the prognostic significance and function of the complement factor I (CFI) in glioma.Materials and Methods: The expression levels of CFI in glioma tissues and the survival of the CFIhigh and CFIlow patient groups were analyzed using The Cancer Genome Atlas (TCGA) database and Genotype-Tissue Expression (GTEx). The correlation between CFI expression and clinicopathological features of glioma was determined by univariate and multivariate Cox regression analyses in the Chinese Glioma Genome Atlas (CGGA) database. The functional role of CFI in glioma was established through routine in vitro and in vivo assays.Results: CFI is overexpressed in glioma and its high levels correlated with poor outcomes in both TCGA and CGGA datasets. Furthermore, CFI was identified as an independent prognostic factor of glioma in the CGGA database. CFI knockdown in glioma cell lines inhibited growth in vitro and in vivo, whereas its ectopic expression increased glioma cell proliferation, migration, and invasion in vitro. CFI protein levels were also significantly higher in the glioma tissues resected from patients and correlated to worse prognosis.Conclusions: CFI is a potential prognostic biomarker in glioma and drives malignant progression.Funding Information
- National Natural Science Foundation of China (81871598)
This publication has 47 references indexed in Scilit:
- Analysis of Binding Sites on Complement Factor I Using Artificial N-Linked GlycosylationOnline Journal of Public Health Informatics, 2012
- Post-radiation increase in VEGF enhances glioma cell motility in vitroRadiation Oncology, 2012
- Complement: a key system for immune surveillance and homeostasisNature Immunology, 2010
- Mutations in complement factor I as found in atypical hemolytic uremic syndrome lead to either altered secretion or altered function of factor IEuropean Journal of Immunology, 2009
- Locally Produced Complement Fragments C5a and C3a Provide Both Costimulatory and Survival Signals to Naive CD4+ T CellsImmunity, 2008
- Complement driven by conformational changesNature Reviews Immunology, 2008
- Gene Expression-Based Molecular Diagnostic System for Malignant Gliomas Is Superior to Histological DiagnosisClinical Cancer Research, 2007
- Malignant astrocytic glioma: genetics, biology, and paths to treatmentGenes & Development, 2007
- Purification and characterization of a membrane protein (gp45-70) that is a cofactor for cleavage of C3b and C4b.The Journal of Experimental Medicine, 1986
- Control of the function of substrate-bound C4b-C3b by the complement receptor Cr1.The Journal of Experimental Medicine, 1984