Ding Chuan Tang Attenuates Airway Inflammation and Eosinophil Infiltration in Ovalbumin-Sensitized Asthmatic Mice
Open Access
- 20 September 2021
- journal article
- research article
- Published by Hindawi Limited in BioMed Research International
- Vol. 2021, 1-10
- https://doi.org/10.1155/2021/6692772
Abstract
Asthma is a T helper 2 (Th2) cell-associated chronic inflammatory diseases characterized with airway obstruction, increased mucus production, and eosinophil infiltration. Conventional medications for asthma treatment cannot fully control the symptoms, and potential side effects are also the concerns. Thus, complement or alternative medicine (CAM) became a new option for asthma management. Ding Chuan Tang (DCT) is a traditional Chinese herbal decoction applied mainly for patients with coughing, wheezing, chest tightness, and asthma. Previously, DCT has been proved to improve children airway hyperresponsiveness (AHR) in a randomized and double-blind clinical trial. However, the mechanisms of how DCT alleviates AHR remain unclear. Since asthmatic features such as eosinophil infiltration, IgE production, and mucus accumulation are relative with Th2 responses, we hypothesized that DCT may attenuate asthma symptoms through regulating Th2 cells. Ovalbumin (OVA) was used as a stimulant to sensitize BALB/c mice to establish an asthmatic model. AHR was detected one day before sacrifice. BALF and serum were collected for immune cell counting and antibody analysis. Splenocytes were cultured with OVA in order to determine Th2 cytokine production. Lung tissues were collected for histological and gene expression analyses. Our data reveal that DCT can attenuate AHR and eosinophil accumulation in the 30-day sensitization asthmatic model. Histological results demonstrated that DCT can reduce cell infiltration and mucus production in peribronchial and perivascular site. In OVA-stimulated splenocyte cultures, a significant reduction of IL-5 and IL-13 in DCT-treated mice suggests that DCT may alleviate Th2 responses. In conclusion, the current study demonstrates that DCT has the potential to suppress allergic responses through the reduction of mucus production, eosinophil infiltration, and Th2 activity in asthma.Funding Information
- Chang Gung Memorial Hospital (BMRP362, CMRPD1K0261~2, CMRPD1K0271~2, NSC 110-2327-B-182-002, NSC 104-2320-B-182-005)
This publication has 57 references indexed in Scilit:
- Xiao-Qing-Long-Tang shows preventive effect of asthma in an allergic asthma mouse model through neurotrophin regulationBMC Complementary and Alternative Medicine, 2013
- Constituents of the anti-asthma herbal formula ASHMI™ synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells, and eotaxin by human lung fibroblasts in vitroJournal of Integrative Medicine, 2013
- Eosinophilic Inflammation in Allergic AsthmaFrontiers in Pharmacology, 2013
- Mast cell engraftment of the peripheral lung enhances airway hyperresponsiveness in a mouse asthma modelAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2012
- Glycyrrhiza uralensis Flavonoids Present in Anti‐Asthma Formula, ASHMITM, Inhibit Memory Th2 Responses in Vitro and in VivoPhytotherapy Research, 2012
- Airway Hyperresponsiveness in Asthma: Mechanisms, Clinical Significance, and TreatmentFrontiers in Physiology, 2012
- Functions of T cells in asthma: more than just TH2 cellsNature Reviews Immunology, 2010
- Mucus hypersecretion in asthma: causes and effectsCurrent Opinion in Pulmonary Medicine, 2009
- The Predictive Value of IgE as Biomarker in AsthmaJournal of Asthma, 2008
- Allergen exposure induces the activation of allergen‐specific Th2 cells in the airway mucosa of patients with allergic respiratory disordersEuropean Journal of Immunology, 1993