Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae
- 22 November 2021
- journal article
- research article
- Published by MyJove Corporation in Journal of Visualized Experiments
- No. 177,p. e63259
- https://doi.org/10.3791/63259
Abstract
Zebrafish larvae possess a fully functional central nervous system (CNS) with a high regenerative capacity only a few days after fertilization. This makes this animal model very useful for studying spinal cord injury and regeneration. The standard protocol for inducing such lesions is to transect the dorsal part of the trunk manually. However, this technique requires extensive training and damages additional tissues. A protocol was developed for laser-induced lesions to circumvent these limitations, allowing for high reproducibility and completeness of spinal cord transection over many animals and between different sessions, even for an untrained operator. Furthermore, tissue damage is mainly limited to the spinal cord itself, reducing confounding effects from injuring different tissues, e.g., skin, muscle, and CNS. Moreover, hemi-lesions of the spinal cord are possible. Improved preservation of tissue integrity after laser injury facilitates further dissections needed for additional analyses, such as electrophysiology. Hence, this method offers precise control of the injury extent that is unachievable manually. This allows for new experimental paradigms in this powerful model in the future.This publication has 24 references indexed in Scilit:
- In vivo Imaging of Mitochondrial Transport in Single-Axon Regeneration of Zebrafish Mauthner CellsFrontiers in Cellular Neuroscience, 2017
- A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for ZebrafishScientific Reports, 2016
- Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafishDevelopment, 2016
- Kidney Regeneration in Adult Zebrafish by Gentamicin Induced InjuryJournal of Visualized Experiments, 2015
- A CRISPR/Cas9 toolkit for multiplex genome editing in plantsBMC Plant Biology, 2014
- Spinal Cord Transection in the Larval ZebrafishJournal of Visualized Experiments, 2014
- Genome Wide Expression Profiling during Spinal Cord Regeneration Identifies Comprehensive Cellular Responses in ZebrafishPLOS ONE, 2014
- Coordinated Development of Voltage-Gated Na+and K+Currents Regulates Functional Maturation of Forebrain Neurons Derived from Human Induced Pluripotent Stem CellsStem Cells and Development, 2013
- A chemical method for fast and sensitive detection of DNA synthesis in vivoProceedings of the National Academy of Sciences of the United States of America, 2008
- Adult zebrafish as a model for successful central nervous system regeneration2008