Expression of small RNAs of Bordetella pertussis colonizing murine tracheas
Open Access
- 1 June 2020
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 64 (6), 469-475
- https://doi.org/10.1111/1348-0421.12791
Abstract
We performed RNA sequencing on Bordetella pertussis, the causative agent of whooping cough, and identified nine novel small RNAs (sRNAs) that were transcribed during the bacterial colonization of murine tracheas. Among them, four sRNAs were more strongly expressed in vivo than in vitro. Moreover, the expression of eight sRNAs was not regulated by the BvgAS two-component system, which is the master regulator for the expression of genes contributing to the bacterial infection. The present results suggest a BvgAS-independent gene regulatory system involving the sRNAs that is active during B. pertussis infection.Funding Information
- Japan Society for the Promotion of Science (17H04075, 19K16638, 19K23845)
- Japan Society for the Promotion of Science
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