Optogenetic and thermogenetic manipulation of defined neural circuits and behaviors in Drosophila
Open Access
- 24 March 2022
- journal article
- review article
- Published by Cold Spring Harbor Laboratory in Learning & Memory
- Vol. 29 (4), 100-109
- https://doi.org/10.1101/lm.053556.121
Abstract
Neural network dynamics underlying flexible animal behaviors remain elusive. The fruit fly Drosophila melanogaster is considered an excellent model in behavioral neuroscience because of its simple neuroanatomical architecture and the availability of various genetic methods. Moreover, Drosophila larvae's transparent body allows investigators to use optical methods on freely moving animals, broadening research directions. Activating or inhibiting well-defined events in excitable cells with a fine temporal resolution using optogenetics and thermogenetics led to the association of functions of defined neural populations with specific behavioral outputs such as the induction of associative memory. Furthermore, combining optogenetics and thermogenetics with state-of-the-art approaches, including connectome mapping and machine learning-based behavioral quantification, might provide a complete view of the experience- and time-dependent variations of behavioral responses. These methodologies allow further understanding of the functional connections between neural circuits and behaviors such as chemosensory, motivational, courtship, and feeding behaviors and sleep, learning, and memory.Keywords
Funding Information
- Japan Society for the Promotion of Science
- Research Fellowship
- KAKENHI (15J06369)
- JSPS Overseas Research Fellowship
- Uehara Memorial Foundation Overseas Postdoctoral Fellowship
- Japanese Biochemical Society Osamu Hayaishi Memorial Scholarship for Study Abroad
- Picower Fellowship
- JPB Foundation
- Massachusetts Institute of Technology Picower Institute for Learning and Memory
This publication has 150 references indexed in Scilit:
- Layered reward signalling through octopamine and dopamine in DrosophilaNature, 2012
- A GAL4-Driver Line Resource for Drosophila NeurobiologyCell Reports, 2012
- Drosophila Evaluates and Learns the Nutritional Value of SugarsCurrent Biology, 2011
- Remembering Nutrient Quality of Sugar in DrosophilaCurrent Biology, 2011
- Presynaptic Facilitation by Neuropeptide Signaling Mediates Odor-Driven Food SearchCell, 2011
- Specific Dopaminergic Neurons for the Formation of Labile Aversive MemoryCurrent Biology, 2010
- A Neural Circuit Mechanism Integrating Motivational State with Memory Expression in DrosophilaCell, 2009
- Comprehensive Maps of Drosophila Higher Olfactory Centers: Spatially Segregated Fruit and Pheromone RepresentationCell, 2007
- The Molecular Basis of Odor Coding in the Drosophila LarvaNeuron, 2005
- Spatial Representation of the Glomerular Map in the Drosophila ProtocerebrumCell, 2002