Assessing Mouse Islet Function
- 17 March 2020
- book chapter
- protocol
- Published by Springer Science and Business Media LLC
- Vol. 2128, 241-268
- https://doi.org/10.1007/978-1-0716-0385-7_17
Abstract
Islets of Langerhans are clusters of endocrine cells embedded within the exocrine pancreas. Islets constitute only approximately 1–2% of the total pancreas mass in all species, so methods have been developed to digest the pancreas and purify islets from the surrounding acinar cells. This chapter provides detailed protocols for isolation of mouse islets and their in vitro functional characterization in terms of assessments of islet viability, hormone content and secretion, second messenger generation and β-cell proliferation.Keywords
This publication has 18 references indexed in Scilit:
- Defining G protein-coupled receptor peptide ligand expressomes and signalomes in human and mouse isletsCellular and Molecular Life Sciences, 2018
- A comparative analysis of human and mouse islet G-protein coupled receptor expressionScientific Reports, 2017
- Impact of islet architecture on β-cell heterogeneity, plasticity and functionNature Reviews Endocrinology, 2016
- New insights into the architecture of the islet of Langerhans: a focused cross-species assessmentDiabetologia, 2015
- Structural similarities and differences between the human and the mouse pancreasIslets, 2015
- The transcriptional landscape of mouse beta cells compared to human beta cells reveals notable species differences in long non-coding RNA and protein-coding gene expressionBMC Genomics, 2014
- Pancreatic islet plasticity: Interspecies comparison of islet architecture and compositionIslets, 2010
- Pancreatic islet cells: A model for calcium‐dependent peptide releaseHFSP Journal, 2010
- Human Pancreatic Islets and Diabetes ResearchJAMA, 2009
- The unique cytoarchitecture of human pancreatic islets has implications for islet cell functionProceedings of the National Academy of Sciences of the United States of America, 2006