Impact of a Rapid Decline in Malaria Transmission on Antimalarial IgG Subclasses and Avidity
Open Access
- 27 January 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Immunology
Abstract
Understanding how immunity to malaria is affected by declining transmission is important to aid vaccine design and understand disease resurgence. Both IgG subclasses and avidity of antigen-specific responses are important components of an effective immune response. Using a multiplex bead array assay, we measured the total IgG, IgG subclasses, and avidity profiles of responses to 18 P. falciparum blood stage antigens in samples from 160 Ugandans collected at two time points during high malaria transmission and two time points following a dramatic reduction in transmission. Results demonstrated that, for the antigens tested, (i) the rate of decay of total IgG following infection declined with age and was driven consistently by the decrease in IgG3 and occasionally the decrease in IgG1; (ii) the proportion of IgG3 relative to IgG1 in the absence of infection increased with age; (iii) the increase in avidity index (the strength of association between the antibody and antigen) following infection was largely due to a rapid loss of non-avid compared to avid total IgG; and (iv) both avid and non-avid total IgG in the absence of infection increased with age. Further studies are required to understand the functional differences between IgG1 and IgG3 in order to determine their contribution to the longevity of protective immunity to malaria. Measuring changes in antibody avidity may be a better approach of detecting affinity maturation compared to avidity index due to the differential expansion and contraction of high and low avidity total IgG.This publication has 83 references indexed in Scilit:
- New Insights into Acquisition, Boosting, and Longevity of Immunity to Malaria in Pregnant WomenThe Journal of Infectious Diseases, 2012
- A Phase 1 Trial of MSP2-C1, a Blood-Stage Malaria Vaccine Containing 2 Isoforms of MSP2 Formulated with Montanide® ISA 720PLOS ONE, 2011
- Antibodies to Plasmodium falciparum Antigens Predict a Higher Risk of Malaria But Protection From Symptoms Once ParasitemicThe Journal of Infectious Diseases, 2011
- Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assayMalaria Journal, 2010
- Immunoglobulin G Subclass-Specific Responses against Plasmodium falciparum Merozoite Antigens Are Associated with Control of Parasitemia and Protection from Symptomatic IllnessInfection and Immunity, 2009
- Pattern of humoral immune response to Plasmodium falciparum blood stages in individuals presenting different clinical expressions of malariaMalaria Journal, 2008
- A semi-automated multiplex high-throughput assay for measuring IgG antibodies against Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) domains in small volumes of plasmaMalaria Journal, 2008
- Breadth and Magnitude of Antibody Responses to MultiplePlasmodium falciparumMerozoite Antigens Are Associated with Protection from Clinical MalariaInfection and Immunity, 2008
- Duration of Naturally Acquired Antibody Responses to Blood-StagePlasmodium falciparumIs Age Dependent and Antigen SpecificInfection and Immunity, 2008
- IgG antibody responses to Plasmodium falciparum merozoite antigens in Kenyan children have a short half-lifeMalaria Journal, 2007