Insight to shape of soil microbiome during the ternary cropping system of Gastradia elata
Open Access
- 4 May 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 20 (1), 1-13
- https://doi.org/10.1186/s12866-020-01790-y
Abstract
Background The ternary cropping system of Gastradia elata depends on a symbiotic relationship with the mycorrhizal fungi Armillaria mellea, which decays wood to assimilate nutrition for the growth of G. elata. The composition of microbe flora as key determinants of rhizoshere and mycorrhizoshere soil fertility and health was investigated to understand how G. elata and A. mellea impacted on its composition. The next generation pyrosequencing analysis was applied to assess the shift of structure of microbial community in rhizoshere of G. elata and mycorrhizoshere of A. mellea compared to the control sample under agriculture process. Results The root-associated microbe floras were significantly impacted by rhizocompartments (including rhizoshere and mycorrhizoshere) and agriculture process. Cropping process of G. elata enhanced the richness and diversity of the microbial community in rhizoshere and mycorrhizoshere soil. Furthermore, planting process of G. elata significantly reduced the abundance of phyla Basidiomycota, Firmicutes and Actinobacteria, while increased the abundance of phyla Ascomycota, Chloroflexi, Proteobacteria, Planctomycetes, and Gemmatimonadetes in rhizoshere and mycorrhizoshere. Besides, A. mellea and G. elata significantly enriched several members of saprophytoic and pathogenic fungus (i.e., Exophiala, Leptodontidium, Cosmospora, Cercophora, Metarhizium, Ilyonectria, and Sporothrix), which will enhance the possibility of G. elata disease incidence. At the same time, the ternary cropping system significantly deterred several members of beneficial ectomycorrhizal fungus (i.e., Russula, Sebacina, and Amanita), which will reduce the ability to protect G. elata from diseases. Conclusions In the ternary cropping system of G. elata, A. mellea and G. elata lead to imbalance of microbial community in rhizoshere and mycorrhizoshere soil, suggested that further studies on maintaining the balance of microbial community in A. mellea mycorrhizosphere and G. elata rhizosphere soil under field conditions may provide a promising avenue for high yield and high quality G. elata.Keywords
Funding Information
- the Key Project at Central Government Level (2060302)
- The National Natural Science Foundation of China (81960694)
- National Key R&D Program of China (2017YFC700703)
- National Technical System of Traditional Chinese Medicine Industry (CARS-21)
- he High-level Innovative Talents of Guizhou Province of China (Qian Ke He Platform and Talent [2018]5638)
- the Science and Technology Project in Guizhou Province of China (Qian Ke He Platform and Talent [2019]5611)
- Guizhou Education Department Innovation Group Major Research Projects (Qian Jiao He KY Zi [2018]022)
- Science and Technology Plan of Guizhou Province (Qian Ke He Zhi Cheng [2018]2764)
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