Blood Bacterial Profiles Associated With Human Immunodeficiency Virus Infection and Immune Recovery
- 1 February 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 223 (3), 471-481
- https://doi.org/10.1093/infdis/jiaa379
Abstract
Human immunodeficiency virus (HIV) infection impairs mucosal immunity and leads to bacterial translocation, fueling chronic inflammation and disease progression. While this is well established, questions remain about the compositional profile of the translocated bacteria, and to what extent it is influenced by antiretroviral therapy (ART). Using 16S ribosomal DNA targeted sequencing and shotgun proteomics, we showed that HIV increases bacterial translocation from the gut to the blood. HIV increased alpha diversity in the blood, which was dominated by aerobic bacteria belonging to Micrococcaceae (Actinobacteria) and Pseudomonadaceae (Proteobacteria) families, and the number of circulating bacterial proteins was also increased. Forty-eight weeks of ART attenuated this phenomenon. We found that enrichment with Lactobacillales order, and depletion of Actinobacteria class and Moraxellaceae and Corynebacteriacae families, were significantly associated with greater immune recovery and correlated with several inflammatory markers. Our findings suggest that the molecular cross talk between the host and the translocated bacterial products could influence ART-mediated immune recovery.Funding Information
- Instituto de Salud Carlos III (PI15/00345, PI18/00154)
- Fundación Asociación Española contra el Cancer
- European Research Area–NET (AC17/00019)
- European Development Regional Fund (PT17/0019)
- Proteomics Facility of the Spanish National Center for Biotechnology)
This publication has 39 references indexed in Scilit:
- Altered CD4+ T cell homing to the gut impairs mucosal immune reconstitution in treated HIV-infected individualsJCI Insight, 2012
- Evidence for Polymicrobic Flora Translocating in Peripheral Blood of HIV-Infected Patients with Poor Immune Response to Antiretroviral TherapyPLOS ONE, 2011
- Plasma Levels of Soluble CD14 Independently Predict Mortality in HIV InfectionThe Journal of Infectious Diseases, 2011
- Late presentation of HIV infection: a consensus definitionHIV Medicine, 2010
- Damaged Intestinal Epithelial Integrity Linked to Microbial Translocation in Pathogenic Simian Immunodeficiency Virus InfectionsPLoS Pathogens, 2010
- Plasma Levels of Bacterial DNA in HIV Infection: The Limits of Quantitative Polymerase Chain ReactionThe Journal of Infectious Diseases, 2010
- Plasma Levels of Bacterial DNA Correlate with Immune Activation and the Magnitude of Immune Restoration in Persons with Antiretroviral‐Treated HIV InfectionThe Journal of Infectious Diseases, 2009
- Microbial translocation is associated with sustained failure in CD4+ T-cell reconstitution in HIV-infected patients on long-term highly active antiretroviral therapyAIDS, 2008
- The mucosal barrier and immune activation in HIV pathogenesisCurrent Opinion in HIV and AIDS, 2008
- Microbial translocation is a cause of systemic immune activation in chronic HIV infectionNature Medicine, 2006