This study was conducted on Pujodadi Village, Trimurjo, Central Lampung from September to December 2020. The study used a factorial randomized block design with two factors and three replications. The first factor is concentration of plant growth substances which consists of 5 levels; Z0 (0 ml.lt-1), Z1 (3.5 ml.lt-1), Z2 (7 ml.lt-1), Z3 (10.5 ml.lt-1) and Z4 (14 ml.lt-1). The second factor is the origin of the seed consists of three parts; P1 (top), P2 (middle), and P3 (bottom). The research objective was to study the effect of plant growth substances concentration and part of seed origin in sugarcane seedlings. The results showed that the plant growth substances concentration significantly increased the percentage of growth and the time 50% of the seeds sprouted. The seed origin of the top part showed the best growth compared to the middle and bottom. There was no interaction between plant growth substances concentration and seed origin on sugarcane seed growth.