Python language, as one of the most popular programming languages, has become the preferred programming course in Colleges and universities. However, in traditional teaching, the dull and monotonous teaching of Python course leads to the low teaching efficiency of Python course and the unsatisfactory learning effect of students. Therefore, there is an urgent need for new teaching methods to improve classroom efficiency. Adopting Python interactive online teaching can not only improve the teaching efficiency of Python course, but also promote the reform of information technology course.