Mass Spectrometry-Based Proteomic Characterization of Cutaneous Melanoma Ectosomes Reveals the Presence of Cancer-Related Molecules
Open Access
- 21 April 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (8), 2934
- https://doi.org/10.3390/ijms21082934
Abstract
Cutaneous melanoma (CM) is an aggressive type of skin cancer for which effective biomarkers are still needed. Recently, the protein content of extracellular vesicles (ectosomes and exosomes) became increasingly investigated in terms of its functional role in CM and as a source of novel biomarkers; however, the data concerning the proteome of CM-derived ectosomes is very limited. We used the shotgun nanoLC–MS/MS approach to the profile protein content of ectosomes from primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cell lines. Additionally, the effect exerted by CM ectosomes on recipient cells was assessed in terms of cell proliferation (Alamar Blue assay) and migratory properties (wound healing assay). All cell lines secreted heterogeneous populations of ectosomes enriched in the common set of proteins. A total of 1507 unique proteins were identified, with many of them involved in cancer cell proliferation, migration, escape from apoptosis, epithelial–mesenchymal transition and angiogenesis. Isolated ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of different cancer-promoting molecules. Taken together, these results confirm the significant role of ectosomes in several biological processes leading to CM development and progression, and might be used as a starting point for further studies exploring their diagnostic and prognostic potential.Funding Information
- Narodowe Centrum Nauki (2013/11/B/NZ4/04315)
This publication has 49 references indexed in Scilit:
- The current status of S-100B as a biomarker in melanomaEuropean Journal of Surgical Oncology, 2012
- CUB-domain–containing protein 1 (CDCP1) activates Src to promote melanoma metastasisProceedings of the National Academy of Sciences of the United States of America, 2011
- Membrane transport proteins in human melanoma: associations with tumour aggressiveness and metastasisBritish Journal of Cancer, 2010
- Serum Amyloid A As a Prognostic Marker in Melanoma Identified by Proteomic ProfilingJournal of Clinical Oncology, 2009
- Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFRProceedings of the National Academy of Sciences of the United States of America, 2009
- Comparative Proteomic Analysis of Matched Primary and Metastatic Melanoma Cell LinesJournal of Proteome Research, 2008
- Human Tumor-Released Microvesicles Promote the Differentiation of Myeloid Cells with Transforming Growth Factor-β–Mediated Suppressive Activity on T LymphocytesCancer Research, 2006
- The human melanoma cell line MelJuSo secretes bioactive FasL and APO2L/TRAIL on the surface of microvesicles. Possible contribution to tumor counterattackExperimental Cell Research, 2004
- The α3β1 Integrin Is Involved in Melanoma Cell Migration and InvasionExperimental Cell Research, 1995
- Integrin alpha 2 beta 1 is upregulated in fibroblasts and highly aggressive melanoma cells in three-dimensional collagen lattices and mediates the reorganization of collagen I fibrils.The Journal of cell biology, 1991