Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry
Open Access
- 1 July 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 6 (30), eaba1195
- https://doi.org/10.1126/sciadv.aba1195
Abstract
Immotile cilia sense extracellular signals such as fluid flow, but whether Ca2+ plays a role in flow sensing has been unclear. Here, we examined the role of ciliary Ca2+ in the flow sensing that initiates the breaking of left-right (L-R) symmetry in the mouse embryo. Intraciliary and cytoplasmic Ca2+ transients were detected in the crown cells at the node. These Ca2+ transients showed L-R asymmetry, which was lost in the absence of fluid flow or the PKD2 channel. Further characterization allowed classification of the Ca2+ transients into two types: cilium-derived, L-R-asymmetric transients (type 1) and cilium-independent transients without an L-R bias (type 2). Type 1 intraciliary transients occurred preferentially at the left posterior region of the node, where L-R symmetry breaking takes place. Suppression of intraciliary Ca2+ transients delayed L-R symmetry breaking. Our results implicate cilium-derived Ca2+ transients in crown cells in initiation of L-R symmetry breaking in the mouse embryo.Funding Information
- Ministry of Education, Culture, Sports, Science, and Technology (17H01435)
- Ministry of Education, Culture, Sports, Science, and Technology (17K15154)
- Ministry of Education, Culture, Sports, Science, and Technology (19K06753)
- Core Research for Evolutional Science and Technology, Japan Science and Technology Agency (JPMJCR13W5)
- National Institute for Basic Biology Individual Collaborative Research Program (19-333)
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