Biocompatibility and Antimicrobial Activity of Reynoutria elliptica Extract for Dental Application
Open Access
- 25 May 2020
- Vol. 9 (6), 670
- https://doi.org/10.3390/plants9060670
Abstract
This study was conducted to determine whether nature-derived Reynoutria elliptica extracts exhibit biocompatibility and antimicrobial effects against oral pathogens such as Streptococcus mutans and Candida albicans. Fine particles of Reynoutria elliptica extract were used to probe for biocompatibility and antimicrobial activity toward these pathogens, and results were evaluated with an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, spectrophotometric growth inhibitory assay, the total number of colony-forming units (CFU), an agar disk diffusion test, and scanning electron microscopy (SEM). In addition, UV/VIS spectroscopy was used to determine the levels of flavonoid and polyphenol in experimental solutions. Several experimental groups showed cell viability higher than 70%, and the antimicrobial activity toward both S. mutans and C. albicans was significantly higher than was that seen for the control group. In CFU and agar disk diffusion tests with C. albicans, increases in the concentration of Reynoutria elliptica extract led to significantly increased antimicrobial effects. Additionally, SEM results showed that Reynoutria elliptica extract changed the morphology and density of S. mutans and C. albicans. The results of this research can be applied to the use of Reynoutria elliptica extracts for the development of oral products that are biologically friendly and can control oral diseases such as dental caries and candida-associated denture stomatitis.Keywords
Funding Information
- National Research Foundation of Korea (2017R1C1B5076310)
This publication has 56 references indexed in Scilit:
- Streptococcus mutans, Candida albicans, and the Human Mouth: A Sticky SituationPLoS Pathogens, 2013
- Antioxidant and Antibacterial Activities of Crude Extracts and Essential Oils of Syzygium cumini LeavesPLOS ONE, 2013
- A review on therapeutic potential of Nigella sativa: A miracle herbAsian Pacific Journal of Tropical Biomedicine, 2013
- Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral DiseasesEvidence-Based Complementary and Alternative Medicine, 2011
- Characterization of Mucosal Candida albicans BiofilmsPLOS ONE, 2009
- The Antibacterial Activity of Plant Extracts Containing Polyphenols against Streptococcus mutansCaries Research, 2007
- Oral pathoses caused by Candida albicans during chemotherapy: Update on development mechanismsOral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 1996
- Effects of prophylactic administration of cefaclor on transient bacteremia after dental extractionEuropean Journal of Clinical Microbiology & Infectious Diseases, 1996
- Effects of oral rinsing with triclosan and sodium lauryl sulfate on dental plaque formation: a pilot studyEuropean Journal of Oral Sciences, 1993
- Studies on dental caries prevention by traditional medicines. Part XIII. Effect of tea polyphenols on glucan synthesis by glucosyltransferase from Streptococcus mutans.CHEMICAL & PHARMACEUTICAL BULLETIN, 1990