Glycolysis Combined with Core Pluripotency Factors to Promote the Formation of Chicken Induced Pluripotent Stem Cells
Open Access
- 6 February 2021
- Vol. 11 (2), 425
- https://doi.org/10.3390/ani11020425
Abstract
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) in vitro. Previously, a lentivirus induction strategy of introducing Oct4, Sox2, Nanog and Lin28 (OSNL) into the iPSC process has been shown as a possible way to produce chicken iPSCs from chicken embryonic fibroblasts, but the induction efficiency of this method was found to be significantly limiting. In order to help resolve this efficiency obstacle, this study seeks to clarify the associated regulation mechanisms and optimizes the reprogramming strategy of chicken iPSCs. This study showed that glycolysis and the expression of glycolysis-related genes correlate with a more efficient reprogramming process. At the same time, the transcription factors Oct4, Sox2 and Nanog were found to activate the expression of glycolysis-related genes. In addition, we introduced two small-molecule inhibitors (2i-SP) as a “glycolysis activator” together with the OSNL cocktail, and found that this significantly improved the induction efficiency of the iPSC process. As such, the study identifies direct molecular connections between core pluripotency factors and glycolysis during the chicken iPSC induction process and, with its results, provides a theoretical basis and technical support for chicken somatic reprogramming.Keywords
Funding Information
- National Natural Science Foundation of China (31772582, 31872341)
- National Key R & D Plan of China (2017YFE0108000, XKYCX19_123)
This publication has 28 references indexed in Scilit:
- Avian-Induced Pluripotent Stem Cells Derived Using Human Reprogramming FactorsStem Cells and Development, 2012
- The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogrammingCell Research, 2011
- Somatic Oxidative Bioenergetics Transitions into Pluripotency-Dependent Glycolysis to Facilitate Nuclear ReprogrammingCell Metabolism, 2011
- Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated CounterpartsPLOS ONE, 2011
- Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined FactorsCell, 2006
- Isolation of Fibroblasts from Chicken EmbryosCold Spring Harbor Protocols, 2006
- PRODUCTION OF GERMLINE CHIMERIC CHICKENS BY TRANSFER OF CULTURED PRIMORDIAL GERM CELLSCell Biology International, 1997
- Germ line chimera produced by transfer of cultured chick primordial germ cells.Cell Biology International, 1995
- Manipulation of Avian Primordial Germ Cells and Gonadal DifferentiationPoultry Science, 1989
- Capacités reproductrices et descendance de poulets ayant subi un transfert de cellules germinales primordiales durant la vie embryonnaireWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 1976