Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum
Open Access
- 19 January 2021
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 16 (1), e0239171
- https://doi.org/10.1371/journal.pone.0239171
Abstract
RIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune responses to control the infection. These animals fail to mount specific lymphoproliferative responses and produce interferon gamma and TNF-alpha that would activate macrophages and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus chromosome encoding this molecule, which was expressed in insect cells and subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation in dogs with leishmaniasis caused by L. infantum.Funding Information
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (573839/2008-5)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (400913/2013-5)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (400063/2016-6)
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2017/10906-8.)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance Code 001)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance Code 001)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance Code 001)
- Fundação de Amparo à Pesquisa do Estado da Bahia (Master Degree Scholarship)
- Fundação de Amparo à Pesquisa do Estado da Bahia (Scientific Initiation Scholarship)
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