Super-resolution nanoscopy by coherent control on nanoparticle emission
Open Access
- 1 April 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 6 (16), eaaw6579
- https://doi.org/10.1126/sciadv.aaw6579
Abstract
Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to -50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot-labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.Funding Information
- National Natural Science Foundation of China (11527901)
This publication has 38 references indexed in Scilit:
- Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxesScience, 2017
- Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuationsNature Communications, 2016
- Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolutionProceedings of the National Academy of Sciences of the United States of America, 2011
- Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)Proceedings of the National Academy of Sciences of the United States of America, 2009
- Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization MicroscopyBiophysical Journal, 2006
- Imaging Intracellular Fluorescent Proteins at Nanometer ResolutionScience, 2006
- Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)Nature Methods, 2006
- Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolutionProceedings of the National Academy of Sciences of the United States of America, 2005
- Subdiffraction resolution in far-field fluorescence microscopyOptics Letters, 1999
- Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopyOptics Letters, 1994