Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq
Open Access
- 9 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-12
- https://doi.org/10.1038/s41598-021-90982-1
Abstract
Purine rich element binding protein A (Purα), encoded by the Purα gene, is an important transcriptional regulator that binds to DNA and RNA and is involved in processes such as DNA replication and RNA translation. Purα also plays an important role in the nervous system. To identify the function of Pura, we performed RNA sequence (RNA-seq) analysis of Purɑ-KO mouse hippocampal neuron cell line (HT22) to analyze the effect of Purα deletion on neuronal expression profiles. And combined with ChIP-seq analysis to explore the mechanism of Purα on gene regulation. In the end, totaly 656 differentially expressed genes between HT22 and Purα-KO HT22 cells have been found, which include 7 Alzheimer’s disease (AD)-related genes and 5 Aβ clearance related genes. 47 genes were regulated by Purα directly, the evidence based on CHIP-seq, which include Insr, Mapt, Vldlr, Jag1, etc. Our study provides the important informations of Purα in neuro-development. The possible regulative effects of Purα on AD-related genes consist inthe direct and indirect pathways of Purα in the pathogenesis of AD.Funding Information
- National Natural Science Foundation of China (81860220, U1503223)
This publication has 67 references indexed in Scilit:
- Complement component C3 and complement receptor type 3 contribute to the phagocytosis and clearance of fibrillar Aβ by microgliaGlia, 2012
- Lipoprotein Lipase Is a Novel Amyloid β (Aβ)-binding Protein That Promotes Glycosaminoglycan-dependent Cellular Uptake of Aβ in AstrocytesOnline Journal of Public Health Informatics, 2011
- Reelin signaling antagonizes β-amyloid at the synapseProceedings of the National Academy of Sciences of the United States of America, 2009
- The common biological basis for common complex diseases: evidence from lipoprotein lipase geneEuropean Journal of Human Genetics, 2009
- Insulin Receptor Dysfunction Impairs Cellular Clearance of Neurotoxic Oligomeric AβOnline Journal of Public Health Informatics, 2009
- Amyloid Precursor Protein Regulates Brain Apolipoprotein E and Cholesterol Metabolism through Lipoprotein Receptor LRP1Neuron, 2007
- Reelin, lipoprotein receptors and synaptic plasticityNature Reviews Neuroscience, 2006
- Modulation of Synaptic Plasticity and Memory by Reelin Involves Differential Splicing of the Lipoprotein Receptor Apoer2Neuron, 2005
- The cytoplasmic domain of the LDL receptor-related protein regulates multiple steps in APP processingThe EMBO Journal, 2002
- Reelin and ApoE Receptors Cooperate to Enhance Hippocampal Synaptic Plasticity and LearningJournal of Biological Chemistry, 2002