Design and Synthesis of a Fluorescent Probe Based on Copper Complex for Selective Detection of Hydrogen Sulfide

Abstract

Design and Synthesis of a Fluorescent Probe Based on Copper Complex for Selective Detection of Hydrogen Sulfide: A novel fluorescence probe NA-LCX was rationally designed and synthesized for the sequential recognition of Cu²⁺ and H₂S by the combination of hydroxyl-naphthalene and diformylphenol groups. The response properties of NA-LCX for Cu²⁺ ions and H₂S with on-off-on manner were investigated by fluorescence emission spectra. A highly selective and sensitive response of complex NA-LCX-Cu²⁺ for H₂S over other competing amino acids was observed with a limit of detection at 2.79μM. The stoichiometry of NA-LCX toward Cu²⁺ ions was determined to be 1:1 by the UV-Vis absorption spectrum, and the coordination configuration was calculated by density functional theory (DFT) calculations. Moreover, probe NA-LCX was applied successfully for the recognition of Cu²⁺ ions and H₂S in living cells.