Characterization of oleosin genes from forage sorghum in Arabidopsis and yeast reveals their role in storage lipid stability
- 16 October 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Planta
- Vol. 254 (5), 1-12
- https://doi.org/10.1007/s00425-021-03744-8
Abstract
Main conclusion Overexpression of forage sorghum oleosin genes in Arabidopsis oleosin-deficient mutant and yeast showed increased germination rate, triacylglycerol content, and protection against lipase-mediated TAG degradation. Abstract Plant lipids are an important source of ration for cattle or other livestock animals to fulfil their energy needs. Poor energy containing green forages are still one of the major sources of food for livestock animals, leaving the animals undernourished. This lowers the milk and meat production efficiency, thereby affecting human consumption. Oleosin, an essential oil body surface protein, is capable of enhancing and stabilizing the lipid content in plants. We identified and functionally characterized three forage sorghum oleosin genes (SbOle1, SbOle2, and SbOle3) in Arabidopsis and yeast. Phylogenetic analysis of SbOle proteins showed a close relationship with rice and maize oleosins. Expression analysis of SbOle genes determined a higher expression pattern in embryo followed by endosperm, while its expression in the non-seed tissues remained negligible. Overexpression of SbOle genes in Arabidopsis ole1-deficient mutants showed restoration of normal germination whereas control mutant seeds showed lower germination rates. Heterologous overexpression of SbOle in yeast cells resulted in increased TAG accumulation. Additionally, the TAG turnover assay showed the effectiveness of SbOle genes in reducing the yeast endogenous and rumen bacterial lipase-mediated TAG degradation. Taken together, our findings not only provide insights into forage sorghum oleosin for increasing the energy content in non-seed organs but also opened up the direction towards implication of oleosin in rumen protection of fodders.Keywords
Funding Information
- Science and Engineering Research Board (CRG/2019/001154)
- DST-INSPIRE Faculty Scheme (DST/INSPIRE/04/2017/000484)
This publication has 45 references indexed in Scilit:
- Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seedsTheoretical and Applied Genetics, 2013
- In Vivo Packaging of Triacylglycerols Enhances Arabidopsis Leaf Biomass and Energy DensityPlant Physiology, 2013
- Lipid metabolism in the rumen: New insights on lipolysis and biohydrogenation with an emphasis on the role of endogenous plant factorsAnimal Feed Science and Technology, 2012
- Biogenesis and functions of lipid droplets in plants: Thematic Review Series: Lipid Droplet Synthesis and Metabolism: from Yeast to ManJournal of Lipid Research, 2012
- Ensembl 2009Nucleic Acids Research, 2008
- A novel role for oleosins in freezing tolerance of oilseeds in Arabidopsis thalianaThe Plant Journal, 2008
- NCBI BLAST: a better web interfaceNucleic Acids Research, 2008
- Protection of fatty acids against ruminal biohydrogenation in cattleEuropean Journal of Lipid Science and Technology, 2007
- The Accumulation of Oleosins Determines the Size of Seed Oilbodies inArabidopsisTHE PLANT CELL ONLINE, 2006
- Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip methodNature Protocols, 2006