Decrease of peripheral blood mucosal-associated invariant T cells and impaired serum Granzyme-B production in patients with gastric cancer
Open Access
- 9 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cell & Bioscience
- Vol. 11 (1), 1-9
- https://doi.org/10.1186/s13578-020-00518-9
Abstract
Mucosal-associated invariant T (MAIT) cells are an invariant T cell subset, which have been reported to play an antimicrobial role in infectious diseases. However, little is known about it in malignant diseases and tumors, especially in gastric cancer (GC). So in this study, we aim to examine the frequency, phenotype, partial functional capacity and clinical relevance of this cells from GC patients' peripheral blood by flow cytometry. It was shown that the frequency of peripheral blood MAIT cells was negatively correlated with their increasing age in healthy adults. Importantly, comparing to the healthy controls (HC), the frequency and the absolute number of MAIT cells from GC patients' peripheral blood with or without chemotherapy were both significantly lower than those. For the phenotype, the proportion of CD4(-)MAIT cell subset in GC patients without chemotherapy was lower than in HC, but higher than in GC patients with chemotherapy. Whereas, the proportion of CD4(-)CD8(+)MAIT cell subset in GC patients without chemotherapy was significantly lower than that in HC. Finally, the level of Granzyme-B (GrB), a molecule associated with MAIT cells was markedly lower in GC patients. But the correlation between the serum levels of GC-associated tumor antigens and the percentages of MAIT cells in GC patients was not observed. In conclusion, our study shows the decreased frequency, changed phenotypes and partial potentially impaired function of MAIT cells in GC patients, suggesting a possible MAIT cell-based immunological surveillance of GC.Funding Information
- Anhui Provincial Natural Science Foundation (1708085QH183)
- Chinese Postdoctoral Science Foundation (2016M590560)
- Wanjiang Scholars Program of Anhui Province of China
- Young Top Talent Support Program of Anhui Medical University
This publication has 35 references indexed in Scilit:
- MAIT Cells Detect and Efficiently Lyse Bacterially-Infected Epithelial CellsPLoS Pathogens, 2013
- T-helper-1-cell cytokines drive cancer into senescenceNature, 2013
- MR1 presents microbial vitamin B metabolites to MAIT cellsNature, 2012
- Cell cycle arrest and apoptosis of OVCAR-3 and MCF-7 cells induced by co-immobilized TNF-α plus IFN-γ on polystyrene and the role of p53 activationBiomaterials, 2012
- Human MAIT and CD8αα cells develop from a pool of type-17 precommitted CD8+ T cellsBlood, 2012
- Clinical Impact of Different Classes of Infiltrating T Cytotoxic and Helper Cells (Th1, Th2, Treg, Th17) in Patients with Colorectal CancerCancer Research, 2011
- Factors predicting progression of gastric intestinal metaplasia: results of a randomised trial on Helicobacter pylori eradicationGut, 2004
- Selection of evolutionarily conserved mucosal-associated invariant T cells by MR1Nature, 2003
- Tag-72, CA 19.9 and CEA as Tumor Markers in Gastric CancerActa Oncologica, 1994