The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes
Open Access
- 1 January 2014
- journal article
- Published by Scientific Research Publishing, Inc. in Advances in Reproductive Sciences
- Vol. 02 (03), 56-67
- https://doi.org/10.4236/arsci.2014.23007
Abstract
We studied the effect of different storage conditions of porcine ovaries (time, temperature) on the characteristics of the follicular fluid, immature oocyte quality, meiotic competence and in vitro fertilization of oocytes. Ovaries were stored for 2, 4 or 6 h at 3 different temperatures (15°C, 25°C or 35°C). As the storage time increased, pH and glucose concentration of the follicular fluid, percentage of live immature oocytes at germinal vesicle stage, oxidative activity and maturation rate decreased. At higher temperatures, pH and glucose concentration decreased, but oxidative activity and oocyte maturation rate increased. Lactate concentration and immature oocyte ROS production increased as storage time and temperature increased. The ovary storage for longer than 2 h at 25°C and 35°C resulted in low pH of the follicular fluid and high ROS level in immature oocytes. Such conditions seem to damage oocytes and impair their meiotic competence. A decrease in the oxidative activity caused by long time and/or low storage temperature may imply a decrease in oocyte vitality. In conclusion, in the porcine species, the transport of ovaries at 25°C and 35°C for 2 h are the best conditions to maintain adequate oocyte quality, meiotic competence and in vitro fertilization rates.Keywords
This publication has 29 references indexed in Scilit:
- Effect of Preserving Condition of Porcine Ovaries on the Development of In Vitro Matured OocytesReproduction in Domestic Animals, 2011
- Reactive oxygen species in bovine oocyte maturation in vitroReproduction, Fertility and Development, 2009
- Oxidative stress and tumor necrosis factor–α–induced alterations in metaphase II mouse oocyte spindle structureFertility and Sterility, 2007
- Regulation of redox metabolism in the mouse oocyte and embryoDevelopment, 2007
- Redox-Induced Apoptosis of Human Oocytes in Resting Follicles In VitroJournal of the Society for Gynecologic Investigation, 2006
- Presence of cumulus cells during in vitro fertilization protects the bovine oocyte against oxidative stress and improves first cleavage but does not affect further developmentZygote, 2005
- Oocyte morphology in dominant and subordinate folliclesMolecular Reproduction and Development, 1994
- Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stressChemical Research in Toxicology, 1992
- Examination of living and fixed gametes and early embryos stained with supravital fluorochromes (Hoechst 33342 and 3,3?-dihexyloxacarbocyanine iodide)Gamete Research, 1986
- The Porcine Ovarian Follicle: I. Selected Chemical Analysis of Follicular Fluid at Different Developmental Stages1Biology of Reproduction, 1976