Combining 10% propolis with carbonated hydroxyapatite to observe the RANKL expression in a rabbit’s alveolar bone
Open Access
- 1 December 2020
- journal article
- Published by Universitas Airlangga in Dental Journal (Majalah Kedokteran Gigi)
Abstract
Background: Periodontitis causes an increased receptor activator level in the nuclear factor-κβ ligand (RANKL), which is one of the inflammatory mediators that plays a role in osteoclastogenesis. The open flap debridement (OFD) technique is the preferred treatment when accompanied by regenerative periodontal treatment using guided tissue regeneration (GTR) and guided bone regeneration (GBR). Carbonated hydroxyapatite is a GBR material that serves as a scaffold and has strong osteoconductive properties for bone regeneration. Propolis is natural product that can decrease osteoclastogenesis in periodontitis by decreasing the RANKL expression. Purpose: To investigate the RANKL expression after open flap debridement by applying carbonated hydroxyapatite to 10% propolis in the alveolar bone of rabbits. Methods: Nine induced-periodontitis rabbits (Oryctolagus cuniculus) were divided into three treatment groups of Group A OFD, Group B OFD followed by the application of carbonated hydroxyapatite, and Group C OFD followed by application of 10% propolis-carbonated hydroxyapatite. Each group was selected one to euthanised on the seventh, 14th and 28th day, respectively, and prepared using histology slides. The data was analysed using a two-way ANOVA followed by a post-hoc LSD test (p<0.05). Results: The RANKL expression in each group showed significant differences (p=0.00; p<0.05) on the seventh, 14th and 28th day. The post-hoc LSD test showed that the RANKL expression in the treatment group with carbonated hydroxyapatite-10% propolis had significant differences (p<0.05) in the intergroup analysis at different time points. Conclusion: Combining 10% propolis with carbonated hydroxyapatite in OFD treatment can decrease the RANKL expression in a rabbit’s alveolar bone.Keywords
This publication has 22 references indexed in Scilit:
- Loading and Release Profile Assay of Carbonated Hydroxyapatite Incorporated with Propolis as Bone Graft MaterialMajalah Obat Tradisional, 2020
- Growth of NIH 3T3 Fibroblast Cells Exposed to Carbonated Hydroxyapatite with Incorporated PropolisIndonesian Journal of Cancer Chemoprevention, 2020
- Evaluation of Antibacterial Potential of Carbonated Hydroxyapatite Combined with Propolis on Porphyromonas gingivalisMajalah Obat Tradisional, 2020
- Effect of carbonated hydroxyapatite incorporated advanced platelet rich fibrin intrasulcular injection on the alkaline phosphatase level during orthodontic relapseAIP Conference Proceedings, 2018
- Porphyromonas gingivalis: An Overview of Periodontopathic Pathogen below the Gum LineFrontiers in Microbiology, 2016
- Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compoundsFood Research International, 2015
- Mechanisms of Bone Resorption in PeriodontitisJournal of Immunology Research, 2015
- Localization of RANK, RANKL and osteoprotegerin during healing of surgically created periodontal defects in sheepJournal of Periodontal Research, 2014
- Porphyromonas gingivalis Lipid A Phosphatase Activity Is Critical for Colonization and Increasing the Commensal Load in the Rabbit Ligature ModelInfection and Immunity, 2014
- Intrabony Defects, Open‐Flap Debridement, and Decortication: A Randomized Clinical TrialThe Journal of Periodontology, 2014