A novel sucrose transporter gene IbSUT4 involves in plant growth and response to abiotic stress through the ABF-dependent ABA signaling pathway in Sweetpotato
Open Access
- 15 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Plant Biology
- Vol. 20 (1), 1-15
- https://doi.org/10.1186/s12870-020-02382-8
Abstract
To maintain sweetpotato (Ipomoea batatas (L.) Lam) growth and yield, sucrose must be transported from the leaves to the roots. Sucrose transporters or carriers (SUTs or SUCs) transport sucrose and are involved in plant growth and response to abiotic stress. However, the mechanisms of SUTs in sweetpotato abiotic stress resistance remains to be determined. In the present study, we cloned a novel IbSUT4 gene; the protein encoded by this gene is localized in the tonoplast and plasma membrane. The plant growth was promoted in the IbSUT4 transgenic Arabidopsis thaliana lines, with increased expression of AtFT, a regulator of flowering time in plants. Over-expression of IbSUT4 in Arabidopsis thaliana resulted in higher sucrose content in the roots and lower sucrose content in the leaves, as compared to the wild-type (WT) plants, leading to improved stress tolerance during seedling growth. Moreover, we systematically analyzed the mechanisms of IbSUT4 in response to abiotic stress. The results suggest that the ABRE-motif was localized in the IbSUT4 promoter region, and the expression of the ABA signaling pathway genes (i.e., ABF2, ABF4, SnRK2.2, SnRK2.3, and PYL8/RCAR3) were induced, and the expression of ABI1 was inhibited. Our dates provide evidence that IbSUT4 is not only involved in plant growth but also is an important positive regulator in plant stress tolerance through the ABF-dependent ABA signaling pathway.Keywords
Funding Information
- National Natural Science Foundation of China Youth Fund (31701357)
- Shandong Agriculture Innovation team (SDAIT-16-01)
This publication has 67 references indexed in Scilit:
- C-terminal armadillo repeats are essential and sufficient for association of the plant U-box armadillo E3 ubiquitin ligase SAUL1 with the plasma membraneJournal of Experimental Botany, 2010
- Sucrose transporters of higher plantsCurrent Opinion in Plant Biology, 2010
- The interface between metabolic and stress signallingAnnals of Botany, 2009
- Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance TransportPlant Physiology, 2008
- Sucrose Transporter StSUT4 from Potato Affects Flowering, Tuberization, and Shade Avoidance ResponsePlant Physiology, 2007
- Arabidopsis Sucrose Transporter AtSUC9. High-Affinity Transport Activity, Intragenic Control of Expression, and Early Flowering Mutant PhenotypePlant Physiology, 2006
- SUGAR SENSING AND SIGNALING IN PLANTS: Conserved and Novel MechanismsAnnual Review of Plant Biology, 2006
- Identification of a Vacuolar Sucrose Transporter in Barley and Arabidopsis Mesophyll Cells by a Tonoplast Proteomic ApproachPlant Physiology, 2006
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Sugar transporters in higher plants – a diversity of roles and complex regulationTrends in Plant Science, 2000