In this study, an analysis of organic fertilizer of an agro-waste (Sesame straw) plus cow dung was carried out using an epigeic earthworm species Eisenia fetida and Perionyx sansibaricus. Sesame straw is abundantly produced after each harvesting of the crop in Kharif season in arid region of tropical India. The compost produced in presence and absence of earthworm exhibit significant (P0.05) changes in physicochemical properties respectively. In control bedding, the values of water holding capacity enhanced significantly (P<0.05) by 1.28 fold, while organic carbon and C/N ratio decreased significantly (P<0.05) by 19.93% and 31.25% respectively after 60 days of composting.Working of E. fetida in the bedding material showed significant (P<0.001) difference in the level of pH, electrical conductivity, water holding capacity, organic carbon, total nitrogen, C/N ratio, available phosphorous and available potassium. After 60 days of working of P. sansibaricus, these physicochemical properties of the bedding substrate also changed significantly (P<0.001). Analysis of vermibed showed a gradual increase in electrical conductivity, water holding capacity, total nitrogen, available phosphorus and available potassium by 1.51, 1.86, 1.95, 1.78 and 1.75 fold respectively. While the values of pH, organic carbon and C/N ratio declined by 9.30%, 41.80% and 71.48% respectively within 60 days of decomposition. Thus, E. fetida and P. sansibaricus can be applied for production of organic fertilizer of sesame chaff plus cow dung to fulfill the requirement of bio-fertilizers for organic farming and agro-waste management in arid environment.