Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial
Open Access
- 12 February 2021
- journal article
- research article
- Published by MDPI AG in Applied Sciences
- Vol. 11 (4), 1666
- https://doi.org/10.3390/app11041666
Abstract
This study assessed the effects of leukocyte-platelet-rich fibrin (L–PRF) on soft tissue healing and the correlation with the local concentration of growth factors (GF) and cytokines in the dental socket of lower third molars. Forty lower-third molars (20 participants) were included in this randomized, double-blinded, split-mouth study. After extractions, randomized sides received alveolar filling with L–PRF on one side and a natural blood clot on the other side. The pain was assessed for up to seven days and soft tissue healing (Landry index) for 14 days post-extraction. Swabs were collected from the surgical sites for GF and cytokine assessment by flow luminometry. Participants reported lower postoperative pain on the sides grafted with L–PRF, which also presented increased tissue healing scores (p < 0.05). There were increased levels of all GFs and several cytokines at the L–PRF site at day one, while vascular endothelial growth factor (VEGF), IL–10, and IL1–RA remained higher throughout for seven days (p < 0.05). VEGF concentration at L–PRF sites correlated positively with the participants’ blood platelet content (ρ = 0.769). PDGF correlated negatively with pain experience on days 2 and 3, and positively with soft tissue healing scores, while FGFb presented a weak correlation with a reduction of pain on day 3. The use of L–PRF improves the soft tissue healing process and decreases postoperative pain after the third molar extractions, which correlates with an increase in the local concentration of growth factors such as PDGF and FGFb.Keywords
Funding Information
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (E-26/202.713/2019)
This publication has 31 references indexed in Scilit:
- Salivary cytokines as biomarkers of periodontal diseasesPeriodontology 2000, 2015
- Effectiveness of cold therapy in reducing pain, trismus, and oedema after impacted mandibular third molar surgery: a randomized, self-controlled, observer-blind, split-mouth clinical trialInternational Journal of Oral & Maxillofacial Surgery, 2015
- Platelet-Rich Plasma and Deproteinized Bovine Bone Matrix in Maxillary Sinus Lift SurgeryImplant Dentistry, 2015
- Advanced Platelet-Rich Fibrin: A New Concept for Cell-Based Tissue Engineering by Means of Inflammatory CellsJournal of Oral Implantology, 2014
- Pain rating schema: three distinct subgroups of individuals emerge when rating mild, moderate, and severe painJournal of Pain Research, 2013
- Platelet-Rich Fibrin is a Bioscaffold and Reservoir of Growth Factors for Tissue RegenerationTissue Engineering, Part A, 2011
- The design and analysis of split‐mouth studies: What statisticians and clinicians should knowStatistics in Medicine, 2009
- The Role of Vascular Endothelial Growth Factor in Wound HealingJournal of Surgical Research, 2009
- Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part III: Leucocyte activation: A new feature for platelet concentrates?Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2006
- APPLICATION OF VISUAL-ANALOGUE SCALES TO THE MEASUREMENT OF FUNCTIONAL CAPACITYRheumatology, 1976