Insight into the Biological Activity of Hennosides—Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead
Open Access
- 26 January 2021
- Vol. 10 (2), 237
- https://doi.org/10.3390/plants10020237
Abstract
Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration ≥ 500 μg/mL. The antioxidant activity of hennosides (concentration ≥100 μg/mL) was determined by FRAP and ABTS assays. At concentration of 500 μg/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 ± 0.08, 0.62 ± 0.28 and 0.35 ± 0.03 mM FeSO4 × 7H2O, and 0.15 ± 0.01, 0.30 ± 0.01 and 0.09 ± 0.01 mM Trolox. Hennosides at 100 μg/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.Keywords
This publication has 33 references indexed in Scilit:
- Du henné d’un tatouage symbolique à une vraie dermatoseArchives de Pédiatrie, 2011
- A Blue Spectral Shift of the Hemoglobin Soret Band Correlates with the Age (Time Since Deposition) of Dried BloodstainsPLOS ONE, 2010
- Allergic contact dermatitis from temporary henna tattooThe Journal of Dermatology, 2009
- TOTAL ANTI-OXIDANT CAPACITY OF CELL CULTURE MEDIAClinical and Experimental Pharmacology and Physiology, 2007
- Development of high-performance liquid chromatographic fingerprint for the quality control of Rheum tanguticum Maxim. ex Balf.Journal of Chromatography A, 2006
- A temporary henna tattoo causing hair and clothing dye allergyContact Dermatitis, 2005
- Role of Oxidant Stress in Lawsone-Induced Hemolytic AnemiaToxicological Sciences, 2004
- Natural ingredients for colouring and stylingInternational Journal of Cosmetic Science, 2002
- Henna causes life threatening haemolysis in glucose-6-phosphate dehydrogenase deficiencyArchives of Disease in Childhood, 2001
- PLASMA PROTECTIVE EFFECT ON RED BLOOD CELLS EXPOSED TO MECHANICAL STRESSASAIO Journal, 1997