H2S induces NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress

Abstract

In current study, we investigated the relationship between hydrogen sulfide (H₂S) and nitric oxide (NO) in the regulation of ascorbate-glutathione (AsA-GSH) cycle in wheat seedlings by water stress. Findings showed that water stress significantly stimulated the production of H₂S and NO, the transcript levels and activities of enzymes in AsA-GSH cycle, as well as malondialdehyde (MDA) production and electrolyte leakage, but significantly decreased AsA/DHA and GSH/GSSG. Meanwhile, water stress significantly decreased plant height and dry biomass. Except MDA and electrolyte leakage, above changes induced by water stress were reversed by H₂S synthesis inhibitor aminooxyacetic acid (AOA) and NO synthesis inhibitor N^G-nitro-L-arginine methyl ester (L-NAME). However, AOA and L-NAME significantly enhanced MDA production and electrolyte leakage, which further decreased plant height and dry biomass of wheat seedlings under water stress. Application of exogenous H₂S donor sodium hydrosulfide (NaHS) to AOA-treated plants and application of exogenous NO donor sodium nitroprusside (SNP) to L-NAME-treated plants reversed above effects of AOA and L-NAME, respectively. Application of L-NAME plus water stress significantly decreased NO production induced by water stress. However, application of L-NAME plus water stress had no obvious influence on H₂S production induced by water stress, while application of AOA plus water stress significantly reduced the production of H₂S and NO induced by water stress. Current findings suggested that H₂S acted upstream of NO in the regulation of AsA-GSH cycle in wheat seedlings by water stress.