Fucoxanthin alters the apelin-13/APJ pathway in certain organs of γ-irradiated mice
Open Access
- 29 April 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Radiation Research
- Vol. 62 (4), 600-617
- https://doi.org/10.1093/jrr/rraa141
Abstract
Apelin-13 and APJ are implicated in different key physiological processes. This work aims at exploring the radioprotective effect of fucoxanthin (FX) on γ-radiation (RAD)-induced changes in the apelin-13/APJ pathway, which causes damage in the liver, kidney, lung and spleen of mice. Mice were administered FX (10 mg kg–1 day–1, i.p) and exposed to γ-radiation (2.5 Gy week–1) for four consecutive weeks. The treatment of irradiated mice by FX resulted in a significant amendment in protein expression of the apelin-13/APJ/NF-κB signalling pathway concurrently with reduced hypoxia (hypoxia-inducible factor-1α), suppressed oxidative stress marker (malondialdehyde), enhanced antioxidant defence mechanisms (reduced glutathione and glutathione peroxidase), a modulated inflammatory response [interleukin-6 (IL-6), monocyte chemoattractant protein-1, IL-10 and α-7-nicotinic acetylcholine receptor) and ameliorated angiogenic regulators [matrix metalloproteinase (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-1), as well as the tissue damage indicator (lactate dehydrogenase) in organ tissues. In addition, there were significant improvement in serum inflammatory markers tumour necrosis factor-α, IL-10, IL-1β and C-reactive protein compared with irradiated mice. The histopathological investigation of the FX + RAD organ tissues support the biochemical findings where the improvements in the tissues’ architecture were obvious when compared with those of RAD. FX was thus shown to have a noticeable radioprotective action mediated through its regulatory effect on the apelin-13/APJ/NF-κB signalling pathway attributed to its antioxidant and anti-inflammatory activity that was reflected in different physiological processes. It could be recommended to use FX in cases of radiation exposure to protect normal tissues.Keywords
This publication has 62 references indexed in Scilit:
- Renal Cortical Lactate Dehydrogenase: A Useful, Accurate, Quantitative Marker of In Vivo Tubular Injury and Acute Renal FailurePLOS ONE, 2013
- Scavenging Capacity of Marine Carotenoids against Reactive Oxygen and Nitrogen Species in a Membrane-Mimicking SystemMarine Drugs, 2012
- Apelin–APJ induces ICAM-1, VCAM-1 and MCP-1 expression via NF-κB/JNK signal pathway in human umbilical vein endothelial cellsAmino Acids, 2012
- Targeting of the Akt-Nuclear Factor-κB Signaling Network by [1-(4-Chloro-3-nitrobenzenesulfonyl)-1H-indol-3-yl]-methanol (OSU-A9), a Novel Indole-3-Carbinol Derivative, in a Mouse Model of Hepatocellular CarcinomaMolecular Pharmacology, 2009
- Involvement of a Stat3 binding site in inflammation-induced enteric apelin expressionAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2008
- Matrix metalloproteinases and their inhibitors in vascular remodeling and vascular diseaseBiochemical Pharmacology, 2008
- Radical Scavenging and Singlet Oxygen Quenching Activity of Marine Carotenoid Fucoxanthin and Its MetabolitesJournal of Agricultural and Food Chemistry, 2007
- Usefulness of lactate dehydrogenase and its isoenzymes as indicators of lung damage or inflammationEuropean Respiratory Journal, 1996
- Regulation of monocyte chemoattractant protein 1 by cytokines and oxygen free radicals in rat hepatic fat-storing cellsGastroenterology, 1996
- A RAPID AND PRECISE METHOD FOR THE DETERMINATION OF UREAJournal of Clinical Pathology, 1960