Tailoring Interleaflet Lipid Transfer with a DNA-based Synthetic Enzyme
Open Access
- 6 May 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 20 (6), 4306-4311
- https://doi.org/10.1021/acs.nanolett.0c00990
Abstract
Lipid membranes, enveloping all living systems, are of crucial importance, and control over their structure and composition is a highly desirable functionality of artificial structures. However, the rational design of protein-inspired systems is still challenging. Here, we have developed a highly functional nucleic acid construct that self-assembles and inserts into membranes, enabling lipid transfer between inner and outer leaflets. By designing the structure to account for interactions between the DNA, its hydrophobic modifications and the lipids, we successfully exerted control over the rate of interleaflet lipid transfer induced by our DNA-based enzyme. Furthermore, we can regulate the level of lipid transfer by altering the concentration of divalent ions, similar to stimuli responsive lipid-flipping proteins.Funding Information
- Engineering and Physical Sciences Research Council
- University of Cambridge
- National Institute of General Medical Sciences (P41-GM104601)
- H2020 European Research Council (647144)
- Division of Materials Research (DMR-1827346)
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