The occurrence of ice-ice disease is among the main issues affecting Kappaphycus production. The abiotic and biotic factors have major contributions to causing this disease. As biotic factors, marine-derived fungi (MDF) are known to be capable of causing the ice-ice disease. In this study, we determined the abundance of MDF from both healthy and ice-ice-infected nutrient-enriched Kappaphycus alvarezii and K. striatus in the seaweed farms of Tongehat, Sibutu, Tawi-Tawi, southern Philippines, following serial dilution procedure using malt extract agar. Results revealed that the abundance of MDF in ice-ice-infected nutrient-enriched K. alvarezii (3.04 x 10^4 CFU g⁻¹) had shown higher significance (p<0.05) when compared with healthy nutrient-enriched K. alvarezii (3.32 x 10^3 CFU g⁻¹). Similarly, the abundance of MDF in ice-ice-infected nutrient-enriched K. striatus (9.31 x 10^3 CFU g⁻¹) also appeared to have higher significance (p<0.01) than healthy nutrient-enriched K. striatus (6.10 x 10^2 CFU g⁻¹). In terms of seaweed species comparison, the abundance of MDF in healthy nutrient-enriched K. alvarezii had a higher significance (p<0.05) than in healthy nutrient-enriched K. striatus, and the abundance of MDF in ice-ice-infected nutrient-enriched K. alvarezii also had higher significance (p<0.05) than ice-ice-infected nutrient-enriched K. striatus. The great number of MDF in ice-ice-infected nutrient-enriched Kappaphycus suggests that MDF may be potential causative agents and have the capability of inducing ice-ice disease in farmed nutrient-enriched Kappaphycus.