Pretreatments of Broussonetia papyrifera: in vitro assessment on gas and methane production, fermentation characteristic, and methanogenic archaea profile
Open Access
- 9 November 2020
- journal article
- research article
- Published by Asian Australasian Association of Animal Production Societies in Animal Bioscience
- Vol. 35 (9), 1367-1378
- https://doi.org/10.5713/ajas.20.0503
Abstract
Objective: The present study was conducted to examine the gas production, fermentation characteristics, nutrient degradation, and methanogenic community composition of a rumen fluid culture with Broussonetia papyrifera (B. papyrifera) subjected to ensiling or steam explosion (SE) pretreatment.Methods: Fresh B. papyrifera was collected and pretreated by ensiling or SE, which was then fermented with ruminal fluids as ensiled B. papyrifera group, steam-exploded B. papyrifera group, and untreated B. papyrifera group. The gas and methane production, fermentation characteristics, nutrient degradation, and methanogenic community were determined during the fermentation.Results: Cumulative methane production was significantly improved with SE pretreatment compared with ensiled or untreated biomass accompanied with more volatile fatty acids production. After 72 h incubation, SE and ensiling pretreatments decreased the acid detergent fiber contents by 39.4% and 22.9%, and neutral detergent fiber contents by 10.6% and 47.2%, respectively. Changes of methanogenic diversity and abundance of methanogenic archaea corresponded to the variations in fermentation pattern and methane production.Conclusion: Compared with ensiling pretreatment, SE can be a promising technique for the efficient utilization of B. papyrifera, which would contribute to sustainable livestock production systems.Keywords
Funding Information
- National Key R&D Program of China (2017YFF0211702)
- Key Program for International S&T Cooperation Projects of China (2016YFE0109000)
- National Natural Science Foundation of China (31802085, 20A180012)
- Central Public-interest Scientific Institution Basal Research Fund (Y2021GH18-2)
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