Evaluating the effect of graphene oxide PEGylation on the properties of chitosan‐graphene oxide nanocomposite scaffold
- 11 May 2022
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part B: Applied Biomaterials
- Vol. 110 (10), 2353-2368
- https://doi.org/10.1002/jbm.b.35082
Abstract
In this study, graphene oxide (GO) was functionalized with polyethylene glycol (PEG) to understand the effect of PEGlayted GO on properties of chitosan-based nanocomposite scaffold. GO was synthesized according to modified Hummer's method and covalently linked to polymeric chains of PEG to produce polyethylene glycolated GO (PGO). Successful preparation of GO and PGO was confirmed by FT-IR and Raman techniques, where the chemical bonding of PEG and GO nanosheets were concluded based on PGOs' lower zeta potential compared to GO. Nanocomposite scaffolds were prepared by adding equal amounts of GO and PGO into 2% (w/v) chitosan (Cs) solutions. The highly porous scaffolds were developed by lyophilization of solutions. Incorporation of GO and PGO into chitosan scaffold network resulted in uniform and spherical pores. Modified samples offered higher porosity and density, indicating adequate scaffold structure. Improvements in the physical properties of prepared chitosan scaffolds were concluded through higher water absorption and retention values. Compressive strength measurement showed 6.33 and 5.5 times improvement respectively for Cs-GO and Cs-PGO samples compared to Cs scaffold. The Cs-GO scaffolds showed minimum susceptibility toward enzymatic degradation and higher degrees of protein adsorption (26% and 23% improvement in value of adsorbed protein respectively for Cs-GO and Cs-PGO compared to Cs scaffold) and biomineral formation on scaffold surface. Also, Cs-PGO sample showed the highest degree of cell viability and lower hemolysis than both Cs and Cs-GO scaffolds. Investigations showed that cell infiltration into scaffold porous structure was more prominent in Cs-PGO scaffolds than in Cs and Cs-GO scaffolds.Keywords
This publication has 46 references indexed in Scilit:
- The synergetic effect of bioactive ceramic and nanoclay on the properties of chitosan–gelatin/nanohydroxyapatite–montmorillonite scaffold for bone tissue engineeringCeramics International, 2014
- Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffoldsDesigned Monomers and Polymers, 2014
- Significance of calcium phosphate coatings for the enhancement of new bone osteogenesis – A reviewActa Biomaterialia, 2014
- Graphene-based nanomaterials for drug delivery and tissue engineeringJournal of Controlled Release, 2014
- Gold Nanorod‐Assembled PEGylated Graphene‐Oxide Nanocomposites for Photothermal Cancer TherapyPhotochemistry and Photobiology, 2013
- General and Biomimetic Approach to Biopolymer-Functionalized Graphene Oxide Nanosheet through Adhesive DopamineBiomacromolecules, 2012
- Biopolymer functionalized reduced graphene oxide with enhanced biocompatibility via mussel inspired coatings/anchorsJournal of Materials Chemistry B, 2012
- Origin of Enhanced Stem Cell Growth and Differentiation on Graphene and Graphene OxideACS Nano, 2011
- The effect of mean pore size on cell attachment, proliferation and migration in collagen–glycosaminoglycan scaffolds for bone tissue engineeringBiomaterials, 2010
- Surface functionalized titanium thin films: Zeta-potential, protein adsorption and cell proliferationColloids and Surfaces B: Biointerfaces, 2006